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| Author(s): |
Camila Ferraz Lucena Monaco
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2017-06-29 |
| Examining board members: |
Angelo Rafael Carpinelli;
Luciana Chagas Caperuto;
Maria Lucia Cardillo Correa Giannella;
Fernanda Ortis;
Hosana Gomes Rodrigues
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| Advisor: | Angelo Rafael Carpinelli |
| Abstract | |
Fatty acids (FA) may influence the process of glucose-induced insulin. The ω3 FA interferes in several physiological processes, and in the pancreatic islets collaborate to decrease the lipotoxicity induced by palmitic acid. Palmitic acid induces deleterious effects in several tissues, as well as in β cells, where it promotes the alteration of the membrane phospholipid composition, the plasma membrane electric potential, and consequently, the process of the insulin granules extrusion. Chronic exposure of β cells to high concentration of palmitic acid is toxic, leading to decreased insulin secretory response, reduced oxidation and uptake of glucose, and an increase in reactive oxygen species (ROS) which, in supraphysiological amounts, will contribute to β-cell failure and death. ROS may be of mitochondrial origin, through the metabolism of nutrients or even from the activation of the enzymatic complex NADPH oxidase, which is modulated by glucose and FA, including palmitic acid. In contrast, ω3 FA exerts anti-inflammatory and antioxidant effects in several systems, contributing to the improvement of lipid profile and peripheral resistance to insulin. The aim of this study was verify the protective possible effect of AG ω3 against the deleterious effects of palmitic acid on pancreatic β cells. Our results shown that the presence of ω3 FA was effective in preventing secretory damage and increase of EROs, also contributing to the maintenance of cell viability and glucose uptake in the islets treated with palmitic acid, playing an important β-cell protective role. (AU) | |
| FAPESP's process: | 12/16374-4 - POSSIBLE PROTECTIVE EFFECTS OF EPA AND DHA AGAINST APOPTOSIS INDUCED BY PALMITATE IN PANCREATIC ISLETS IN CULTURE |
| Grantee: | Camila Ferraz Lucena Monaco |
| Support Opportunities: | Scholarships in Brazil - Doctorate |