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| Author(s): |
Ismael Pretto Sauter
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2017-11-21 |
| Examining board members: |
Mauro Javier Cortez Véliz;
Silvia Beatriz Boscardin;
Danilo Ciccone Miguel;
Eduardo Lani Volpe da Silveira
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| Advisor: | Mauro Javier Cortez Véliz |
| Abstract | |
L. (L.) amazonensis evades the immune response of host macrophage inducing the CD200 expression in the cell. However, it is not yet known how this mechanism occurs. The objective of this work was to evaluate the participation of TLRs in CD200 induction in infected macrophages by L. (L.) amazonensis. The results showed that the CD200 induction by the parasite is dependent on TLR9 and the adaptor proteins MyD88 and TRIF. In addition, we observed that the CD200 can be induced by the parasite DNA, as well as by extracellular vesicles (EVs) containing DNA released by it. In vivo results showed that the absence of TLR9 does not alter the lesion size nor the CD200 expression in macrophages present in the lesion. However, the parasite load was higher in wild type mice. Therefore, we can conclude that the CD200 induction by L. (L.) amazonensis amastigotes is TLR dependent and this can be stimulated by the parasite DNA. (AU) | |
| FAPESP's process: | 13/14087-0 - Study of the role of Toll-like receptors (TLRs) on CD200 induction in murine macrophages infected with Leishmania (L.) amazonensis |
| Grantee: | Ismael Pretto Sauter |
| Support Opportunities: | Scholarships in Brazil - Doctorate |