The liquid crystal precursor system associated with terpinen-4-ol and nystatin: characterization, antifungal, synergistic action, cytotoxicity and adhesion in oral cells

Author(s)

Renata Serignoli Francisconi

• Document type: Doctoral Thesis
• Press: Araraquara. 2018-04-27.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara
• Defense date: 2018-03-19
• Advisor: Denise Madalena Palomari Spolidorio

Abstract

The liquid crystal precursor systems associated with plant extracts and phytochemical compounds open new perspectives for efficient prevention and control of infectious fungal diseases. The objectives of this study were: I) to evaluate the synergistic effect of the combination of terpinen-4-ol (t-4-ol) with nystatin (nys); II) to evaluate in liquid crystal form the antifungal property of t-4-ol and possible synergism with nys; III) to evaluate the cytotoxic potential of t-4-ol in normal immortalized oral cells (NOK) and to verify whether t-4-ol interferes with the adhesion of Candida albicans to oral cells. Thus, the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (CFM) of t-4-ol on clinical isolates of C. albicans (Genotype A and B) and strain SC 5314 were determined using the microdilution method in broth. Biofilms were prepared using the static microtiter plate model and quantified by colorimetric methodology of the tetrazolium salt reduction assay (XTT). The same tests were applied to evaluate t-4-ol and nys in liquid crystal form. In addition, the cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) medium supplemented with 10% fetal bovine serum and 1% penicillin, streptomycin and glutamine and maintained in an incubator at 37 ° C in 5% CO2 . Linear cytotoxicity of NOK cells was assessed by flow cytometry and the coculture test was performed to verify the interference of t-4-ol on Candida albicans adhesion on oral cells (NOK). Candida albicans adhesion on epithelial cells was evaluated in colony forming units (UFC mL-1) and showed that t-4-ol associated or not with SPCL reduced C. albicans adhesion on epithelial cells. The components presented antifungal action in both planktonic and biofilm culture, in addition, a synergistic effect of the tested components was observed. The components were not cytotoxic at application times of 4 and 8 hours. This study demonstrated that t-4-ol alone or in combination with nys may provide an effective clinical application and when associated with the liquid crystal precursor system induces better efficacy at lower concentrations with control of resistant microorganisms (AU)

• FAPESP's process: 14/22220-5 - Characterization, synergism, antibiofilm and cytotoxicity of nanoparticles loaded with Terpinen-4-ol on C. albicans isolates
• Grantee: Renata Serignoli Francisconi
• Support Opportunities: Scholarships in Brazil - Doctorate