Post-transcriptional regulation of osteoglycin by miR-22 during myogenesis

Author(s)

Carlos Augusto Barnabé Alves

• Document type: Master's Dissertation
• Press: Botucatu. 2016-09-16.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu
• Defense date: 2016-07-26
• Advisor: Maeli Dal Pai; Robson Francisco Carvalho

Abstract

In mammals, myogenesis is the process of embryonic development of muscle tissue at the molecular level that is regulated by interaction of intracellular signal transducers and nuclear transcription factors. Somite cells are committed to the myogenic lineage and progress along the development of muscle cells and subsequently formation of multinucleated myofiber. During these processes there is the activation of Myogenic Regulatory Factors (MRFs), resulting in a reprogramming of gene expression responsible for the control of skeletal myogenesis. MRFs are important for muscle development, but they do not explain by themselves the sophisticated pattern of gene expression during myogenesis. The Osteoglycin (OGN) is part of the small proteoglycans that are secreted by the extracellular matrix and presenting gene expression changes during the myogenesis process. Although OGN has been identified as part of the muscle cell secretome, it has not been rated it role in proliferation, migration and, differentiation of muscle cells. The complexity of the control mechanisms of gene expression in this process suggests the involvement of additional regulatory molecules, such as micro-RNAs. These RNA molecules encoded by the genome regulate various cellular processes in skeletal muscle and are involved in various diseases. Micro-RNAs work in order to control a common biological pathway or function. Using computer algorithms, we found miR-22 as an important candidate for post-transcriptional regulation of OGN. Thus, the hypothesis is that the OGN is post-transcriptionally regulated by miR-22 for the control of proliferation, migration and differentiation of muscle cells during myogenesis. (AU)

• FAPESP's process: 14/21110-1 - The role of miR-22 in the regulation of ostoglycin during myogenesis
• Grantee: Carlos Augusto Barnabe Alves
• Support Opportunities: Scholarships in Brazil - Master