Acute sepsis caused by Aeromonas hydrophila IN Piaractus mesopotamicus: morphological and microbiological characterization

Author(s)

Fausto de Almeida Marinho Neto

• Document type: Master's Dissertation
• Press: Jaboticabal. 2016-12-21.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2016-10-28
• Advisor: Julieta Rodini Engrácia Moraes

Abstract

Aeromonas bacteria cause infection characterized by septicaemia and acute septicemic form of this disease is the most prevalent in fish. This bacteriosis is responsible for high morbidity and mortality rates, causing considerable losses in the aquaculture sector. Thus, understanding its pathophisiology is crutial to the development of strategies for intervention and control of fish aeromonosis. This study aimed to characterize by morphological and microbiological studies the acute phase of septic process in different tissues of pacu (Piaractus mesopotamicus) experimentally infected with Aeromonas hydrophila. Hence, 160 pacus weighing approximately 250g were inoculated via coelomic route with A. hydrophila (1.78 x 109 CFU / mL) and pre-operative times of 0 (control), 1, 3, 6, and 9 hours post-inoculation (hpi) the animals were anesthetized and the biological material collected for performing microbiological, histopathological and ultrastructural analysis. They showed the presence of necrosis, degenerative processes, vascular damage and close association of the bacteria to these lesions in tissues, mainly 6 and 9 hours after inoculation. (AU)

• FAPESP's process: 15/01024-6 - Structural and ultrastructural characterization of infection by Aeromonas hydrophila in Piaractus mesopotamicus
• Grantee: Fausto de Almeida Marinho Neto
• Support Opportunities: Scholarships in Brazil - Master