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Structural and ultrastructural characterization of infection by Aeromonas hydrophila in Piaractus mesopotamicus

Grant number: 15/01024-6
Support type:Scholarships in Brazil - Master
Effective date (Start): May 01, 2015
Effective date (End): August 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Julieta Rodini Engracia de Moraes
Grantee:Fausto de Almeida Marinho Neto
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Among the problems in fish farming stand out the bacterial diseases which are installed due to stress caused by environmental adverse conditions and handling which are responsible for slow growth and high mortality rates. Techniques such as light microscopy and Transmission Electron Microscopy (TEM) are used in the study of biological material, in particular, to examine structural changes, ultrastructural and cytopathic effects caused by bacteria and other microorganisms in several types of infections. This diagnostic procedure contributes to understand the host-pathogen interaction, which is very important to the understanding of the pathogenesis and development of strategies for intervention and disease control. This project aims to investigate sepsis through the study of infection, dissemination and colonization of A. hydrophila in blood and different organs of pacus (Piaractus mesopotamicus) and the serum lytic activity. For this, it will be used 170 pacus (weight 150.71 ± 56,73g and total length 18.27 ± 4.70 cm). Initially, we will determine the LD50 (LD50-96h) for A. hydrophila in pacus. Sepsis will be induced by administration of celomatic inoculum corresponding to the LD50 and a group injected by the same route with 0.65% saline sterile as a control. Before inoculation and 1, 3, 6 and 9 hours after inoculation, ten animals in each group (saline control and challenged) will be anesthetized for blood collection, necropsy and organ collection for light microscopy and transmission electron microscopy analysis, dissemination of the bacteria in the different compartments and to chart the growth curve of A. hydrophila. Throughout the experiment, the animals will be evaluated for clinical behavior changes. The results will be compared by analysis of variance (ANOVA) at 5% probability and the difference between means by Tukey test (p <0.05). (AU)

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
NETO, Fausto de Almeida Marinho. Sepse aguda por Aeromonas hydrophila EM Piaractus mesopotamicus : características morfológicas e microbiológicas. 2016. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias..

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