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| Author(s): |
Silvelise Pupin
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Ilha Solteira. 2018-02-26. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Engenharia. Ilha Solteira |
| Defense date: | 2018-02-02 |
| Advisor: | Mario Luiz Teixeira de Moraes; Celso Luis Marino |
| Abstract | |
The success of forest breeding programs requires that the prediction of genetic progress be proportionate to actual genetic progress. For this, it is possible to join the quantitative genetics with molecular tools, which allow to estimate parameters with greater acuity for selection. The main was to verify the genetic diversity, mating system and kinship relationships in open pollinated populations of Eucalyptus urophylla, to select crosses and parents. In order to do, two seedlings seed orchards (SSO) and an adult sample of individuals were used in a progeny test (PCPN). To compose a second generation progenies test (2GPT) seeds were collected in 23 mother trees in one of the SSO. The populations are located in the Fazenda de Ensino, Pesquisa e Extensão, in Selvíria-MS. The individuals from 2GTP (722) and adults (467) were genotyped with 12 microsatellite markers (SSR) and the loci evaluated for genetic markers. Silvicultural traits were measured in the SSO and 2GPT. SSR markers formed a consistent set of genetic markers. The genetic diversity of the populations was high and the reduction was small between generations. The fixation index was significant for all populations and higher in the offspring, indicating the presence of inbreeding. The multilocus estimate of outcrossing rate indicated that reproduction occurred predominantly by crosses (0.964). There were crosses between relatives, crosses correlated and self-fertilization. As a result, inbreeding in the progenies was higher than in the parental population and the main reason was cross between relatives. In addition, kinship relations were higher in 2GPT. There was pollen flow among the populations and the number of pollen donors was higher by paternity analysis, which allowed to determine the probable candidate father of 713 individuals of the 2GPT. Thus, it was possible to study the open pollinated progeny test as a full sib test, allowing the estimation of general and specific combining abilities. For the diameter at breast height, the genetic gain based on the genotypic value of the cross was high (> 20%), as well as the clonal selection, which indicated considerable gains (> 65%). Thus, it is possible to continue the Breeding Program, indicating promising crosses and genotypes of E. urophylla. (AU) | |
| FAPESP's process: | 14/03407-7 - Genetic diversity, mating system and parentage in progenies of eucalyptus urophylla st Blake |
| Grantee: | Silvelise Pupin |
| Support Opportunities: | Scholarships in Brazil - Doctorate |