Effect of Roux-En-Y gastric bypass on liver of male offspring of cafeteria obese rats

Author(s)

Mariana Carla Mendes

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
• Defense date: 2018-03-09

Examining board members

Antonio Carlos Boschero; Marciane Milanski; Camila Aparecida Machado De Oliveira; Antonio Marcus de Andrade Paes; Patrícia Cristina Lisboa

• Advisor: Antonio Carlos Boschero; Emerielle Cristine Vanzela; Maria Lúcia Bonfleur

Abstract

Obesity is an important public health problem with high prevalence in adult as well as young people. Alterations in maternal nutrition and metabolism can affect the offspring in a stable way, a phenomenon defined as fetal programation. It is also demonstrated that paternal obesity also increases the risk to develop co morbidities, related to this syndrome, in the offspring. Obesity and co-morbidities can be reverted and also avoided with the reduction of body weight. Bariatric surgery is one of the most powerful treatments of these problems since they are able to induce weight loss, reducing the risk derived from obesity. Among the bariatric surgeries, the Roux-en-Y gastric bypass (RYGB) is the gold standard technique for the treatment of obese diabetic patients. However, it was not demonstrated, until now, if the weight loss and the metabolic amelioration, observed after such a paternal surgery, may prevent the deleterious effects upon metabolic parameters in the offspring. Here, we analyzed the effects of the paternal bariatric surgery (DGYR) on liver parameters of offspring aged 1 month. For this purpose, 2 months old male Wistar rats received cafeteria diet (CAF) for 10 weeks. Then, they are subjected to surgery DGYR (CAF DGYR group) or pseudo-surgery (CAF PC group). After 10 weeks, males of both groups mate with control female (fed with standard diet). At euthanasia¿s day of the rats blood samples were collected for measurement of fast glycemia, insulinemia, triglyceridemia and cholesterolemia. In addition, liver fragments were collected for the analysis of lipid content, genic expression and histology. Males¿ offspring were feed a control diet and euthanized at 1 month of age. The same parameters described above were measured in the offspring. In the father, the bariatric surgery reduced body weight, fat accumulation and food intake, confirming data from literature. In addition, liver fat accumulation and the expression of genes related to "de novo" lipogenesis were reduced. At 1 month of age, the offspring from fathers subjected to DGYR displayed lower concentrations of serum and hepatic triglycerides and cholesterol, although they present higher body weight, compared with offspring of the PC group (AU)

• FAPESP's process: 15/11304-6 - Glycemic and lipid homeostasis of offspring from obese rats subimitted to Roux-en-Y gastric bypass
• Grantee: Mariana Carla Mendes Brandão
• Support Opportunities: Scholarships in Brazil - Doctorate