Influence of electric stimulation and enamel matrix protein derivative application on palatal wound healing: two randomized clinical trials

Author(s)

Manuela Maria Viana Miguel

• Document type: Master's Dissertation
• Press: São José dos Campos. 2020-01-29.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos
• Defense date: 2019-12-16
• Advisor: Mauro Pedrine Santamaria

Abstract

Surgical periodontal procedures may favor the formation of wounds in the palatal region as for example to obtain autogenous graft. Discomfort and a certain degree of morbidity may be present in patients undergoing this procedure. Thus, treatments for the palatine donor area has been sought to aid wounds healing and eventually be useful for other functions. Therefore, the aim of the investigations was to evaluate the 3-month clinical and immunological outcomes of two approaches, electric stimulus (EE) and enamel matrix derivative (EMD), on palatal open wound healing due to harvesting a graft for socket preservation. For this, two clinical trials were carried out following the CONSORT STATEMENT 2010. (1) Fifty-three patients presenting need of ridge preservation were divided into 2 groups: Sham (n=27) - sham electrical stimulation on the open palatal wound; EE (n=26) - electrical stimulation on open palatal wound. Clinical perceptions shown early wound closure, as well as, better epithelization in 7 and 14 days after surgery in EE group when related to Sham group (p<0,05 e p=0,03, respectively). Painful symptomatology was less reported in EE group in comparison to Sham group at 3 days after surgical approach (p=0,008). Likewise, an improvement in Oral Health Impact Profile was reported after 2 days of the procedure when combinate with electrotherapy protocol (p<0,04). Biomarkers modulation occurred in a favor manner when electric stimulus was applied at the wound. Therefore, the use of electrotherapy presented benefits for clinical wound healing and influenced the expression of favorable biomarkers during injury resolution. (2) Forty-four patients presenting need of ridge preservation were allocated into 2 groups: Control (n=22) - open palatal wound without treatment and EMD (n=22) – Emdogain® applied on the open palatal wound. None clinical benefits, as wound closure and epithelization was seen when EMD was used. However, important biomarkers modulation for repair occurred, as well as, less painful symptomatology. In conclusion, EMD application shown a positive influence in local inflammation during wound repair. (AU)

• FAPESP's process: 18/03353-5 - Influence of electric stimulation on palatal wound healing: randomized clinical trial
• Grantee: Manuela Maria Viana Miguel
• Support Opportunities: Scholarships in Brazil - Master