Effect of interleukin 17 on hypothalamic regulation of energy homeostasis

Author(s)

Guilherme Augusto da Silva Nogueira

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas
• Defense date: 2019-08-14

Examining board members

Licio Augusto Velloso; Luiz Osório Leiria; Adriana Souza Torsoni; Joana Margarida Navalho Gaspar; Andreza Fabro de Bem

• Advisor: Licio Augusto Velloso

Abstract

Obesity results from the association of genetic, metabolic, hormonal and environmental factors. Once established, it favors the development of insulin resistance, cardiovascular diseases, hypertension, dyslipidemia and some types of cancer, impacting on life quality and mortality. Due to its huge impact on the economics of public health and because its prevalence is currently about 650 million people, obesity has grown to become one of the most important clinical and epidemiological problems in the world. Recent studies have provided evidence that interleukin-17 (IL-17) is associated with obesity. IL-17 is produced in the gut in response to changes in the microbiome landscape and food intake, thus, it is possible that it is involved in central the regulation of feeding. Here, we employed real-time PCR, immunoblot, immunofluorescence, metabolic assays, thermographic images, measurement of neurotransmitters to evaluate the actions of IL-17 in C57BL/6 and reporter mice. IL-17 receptor A (IL-17RA) was detected in the hypothalamus, particularly POMC, NPY, AgRP and MCH neurons. Moreover, the intraperitoneal (IP) and intracerebroventricular (ICV) injections of IL-17 resulted in increased POMC expression, which was accompanied by reduced food intake. The IP injection of IL-17 stimulated the phosphorylation of MEK4 and STAT3 in the hypothalamus, increased the brown adipose tissue temperature and its ICV injection resulted in reduction of respiratory quotient. Taken together the results of this original study demonstrate that IL-17 exerts an important function in the regulation of the hypothalamic energy homeostasis and thermogenesis actions (AU)

• FAPESP's process: 14/24362-1 - Effect of interleukin 17 in the hypothalamic regulation of energy homeostasis
• Grantee: Guilherme Augusto da Silva Nogueira
• Support Opportunities: Scholarships in Brazil - Doctorate