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| Author(s): |
Renato Bueno Flores
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2019-06-28 |
| Examining board members: |
Camila Infantosi Vannucchi;
Daniel de Souza Ramos Angrimani;
Cristina de Fatima Lucio
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| Advisor: | Camila Infantosi Vannucchi |
| Abstract | |
The objective of the present study is to analyze the influence of Benign Prostatic Hyperplasia (BPH) on the main freezing parameters of canine sperm. For such purpose, 20 dogs were previously selected and composed 3 experimental groups, according to the presence or absence of BPH diagnosis and treatment with finasteride: control group (n=9), BPH group (n=5) and BPH+FIN (n=6). Complementarily, the experiment was subdivided in fresh or post-thaw sperm. Dogs had more than 6 years and a presumptive diagnosis of BPH through the verification of clinical signs, rectal examination and B mode ultrasonography. The fresh seminal samples were evaluated for volume, color, aspect, sperm concentration, sperm motility, sperm mitochondrial activity, oxidative stress, plasmatic and acrosomal membrane integrity, sperm DNA fragmentation by flow citometry and sperm binding test on perivitelic membrane of chicken egg yolk. Subsequently, semen was cryopreserved in one step, thawed at 37°C for 30 seconds and evaluated according to previously described. For the fresh semen results, the BPH+Finasteride group had lower score of ejaculate aspect compared to the other groups. Additionally, BPH+Finasteride group had a higher frequency of sperm flagellar beating in relation to the control group and a lower percentage of sperm with medium velocity in relation to the other groups. The control group had higher percentage of medium sperm mitochondrial activity in relation to the other groups. Also, the control group had higher percentage of sperm DNA integrity. Conversely, BPH group showed the lowest percentage of DNA integrity. For the post-thaw analysis, the control group presented a higher percentage of high mitochondrial activity compared to the other groups. However, the BPH+Finasteride group showed a higher number of spermatozoa bound to the perivitelinic membrane of chicken egg yolk, compared to the BPH group. The BPH group presented a higher percentage of spermatozoa with acrossomal membrane lesion (without lesion of plasmatic membrane) compared to the control group. Regarding the oxidative stress, the control group presented higher lipid peroxidation compared to the BPH+Finasteride group. On the other hand, the control group had higher sperm DNA integrity compared to the other groups, and the BPH group had the lowest percentage of sperm DNA integrity. In conclusion, sperm of BPH dogs presented higher susceptibility to cryoinjury. However, finasteride treatment was able to minimize the deleterious effects of sperm cryopreservation, allowing for the use of HPB dogs for reproductive purposes. (AU) | |
| FAPESP's process: | 17/05492-0 - The influence of benign prostatic hyperplasia and treatment with finasteride on the main freezing parameters of canine spermatozoa |
| Grantee: | Renato Bueno Flores |
| Support Opportunities: | Scholarships in Brazil - Master |