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Influence of benign prostatic hyperplasia and treatment with finasteride on sperm DNA integrity in dogs

Grant number: 17/04102-3
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): April 17, 2017
Effective date (End): September 28, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Camila Infantosi Vannucchi
Grantee:Daniel de Souza Ramos Angrimani
Supervisor abroad: Ann Van Soom
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Ghent University (UGent), Belgium  
Associated to the scholarship:13/25966-5 - Characterization of seminal and hemodynamic changes in the prostate and testes of dogs with benign prostatic hyperplasia , BP.DR

Abstract

Benign prostatic hyperplasia (BPH) is one of the most common diseases in canine and human males. However, some details regarding the etiopathogeny of BPH remain unclear, such as the impact of the disease on seminal quality. Moreover, the use of finasteride for the treatment of BPH in men and dogs has not yet been fully established due to the possible side effects on fertility. In humans, it is known BPH and the use of finasteride may lead to sperm alterations, such as impaired sperm DNA integrity. In dogs there are few studies focusing on the influence of the disease and consequent treatment on sperm DNA status. Therefore, the aim of this study is to characterize modifications on DNA integrity related to BPH in dogs submitted to treatment with finasteride. For this purpose, we will select twenty dogs which will be allocated to the following 2x2 factorial design: dogs affected by BPH (n = 5), dogs affected by BPH and treated with finasteride (HPB + F - n = 5), dogs free from BPH (CON - n=5), and dogs treated with finasteride (CON + F - n = 5). All evaluations will be performed at monthly intervals for 60 days starting at the beginning of therapy or diagnosis (D0, D30 and D60). Semen samples will be evaluated for the sperm DNA susceptibility to fragmentation by the Sperm Chromatic Structure Assay (SCSA) and direct evaluation of DNA fragmentation by TUNEL, chromatin dispersion test and chromomycin A3. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANGRIMANI, DANIEL S. R.; BRITO, MAIRA M.; RUI, BRUNO R.; NICHI, MARCILIO; VANNUCCHI, CAMILA I. Reproductive and endocrinological effects of Benign Prostatic Hyperplasia and finasteride therapy in dogs. SCIENTIFIC REPORTS, v. 10, n. 1 SEP 9 2020. Web of Science Citations: 1.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.