Trinuclear -oxo Ruthenium Complex containing N-heterocyclic ligands and Carbon Monoxide: Syntheses, Characterization and Studies on the Interaction with Biomolecules.

Author(s)

Mariete Barbosa Moreira

• Document type: Doctoral Thesis
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC)
• Defense date: 2016-07-28

Examining board members

Sofia Nikolaou; José Maurício Almeida Caiut; Adelino Vieira de Godoy Netto; Roberto Santana da Silva; Vagner Roberto de Souza

• Advisor: Sofia Nikolaou

Abstract

This work describes the synthesis and characterization of four ruthenium trinuclear complex of general formula [Ru3O(CH3COO)6(CO)(L)2] where L = 2,6-dimethylaminopyridine; isonicotinamide; 4-aminopyridine and 4-dimethylpyrazine (N-heterocyclic ligands with -acceptor or -donor characteristics). The ruthenium derivatives were synthesized according to adaptations of synthetic routes already reported in literature and characterized by spectroscopic and electrochemical techniques such as nuclear magnetic ressonance (NMR), UV-vis, infrared, cyclic voltammetry, X-ray diffraction and elemental analysis. The photoinduced release of carbon monoxide in acetonitrile was investigated by means of spectrophotometric monitoring. The quantum yield involved in the CO release was calculated by chemical actinometry. It was studied the interaction between the complexes with the biomolecules HSA and calf thymus DNA by spectroscopic techniques of fluorescence, UV-vis and circular dichroism. In addition, preliminary tests were performed in vitro in order to evaluate the cytotoxicity of the complexes against a murine melanoma cell line (B16F10) and trypanocidal activity of the complexes over the amastigote form of the parasite Trypanosoma cruzi. (AU)

• FAPESP's process: 12/22688-1 - Trinuclear µ-Oxo Ruthenium Complexes with nitrosil and À-acceptor ligands: Synthesis, characterization and interaction studies with biomolecules.
• Grantee: Mariete Barbosa Moreira
• Support Opportunities: Scholarships in Brazil - Doctorate