Effect of topical PTH added to BioGran® on peri-implant defect in rats submitted to orchiectomy

Author(s)

Pedro Henrique Silva Gomes Ferreira

• Document type: Doctoral Thesis
• Press: Araçatuba. 2020-08-24.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2020-08-03
• Advisor: Roberta Okamoto; Paulo Noronha Lisboa Filho; Paulo Roberto Botacin

Abstract

Objective: To evaluate PTH 1 34 in a topical way, functionalized to BioGran® by the sonochemistry technique, in peri implant d efects in rats submitted to orchiectomy. Materials and Methods: 128 rats were divided into 2 groups: SHAM fictitious surgery, ORQ orchiectomy, which each group was subdivided into 3 CLOT groups (periimplant defect, without biomaterial); BG (peri impla nt defect BioGran®); BGPTH (peri implant defect BioGran® functionalized with PTH 1 34). Each animal received an implant in the tibial metaphysis. At 14 and 42 days after surgery, calcein and alizarin fluorochromes were administered, respectively. Eutha nasia occurred at the 30th (ultrastructural and EDX analysis) and 60th day (other analyzes) after the implant installation. The samples were analyzed by immunohistochemistry ( Beta Catenin, TRAP and OC), HE and RT PCR ( ALP and OC), in addition t o biomechanical analysis, microCT for evaluation of bone volume percentage parameters BV/TV), trabecular thickness (Tb. number and separation of trabeculae (Tb. Tb.Sp) and percentage of total porosity, (Po tot) and Bone Implant Contact ( by iS). For laser confocal microscopy, the measurement of the fluorochrome area, the mineral apposition rate ( and the area of ne oformed bone area ( and elemental analysis ( At the ultrastructural analysis MET analyzes were performed. Nonparametric data were compared using the Kruskal Wallis test and parametric data using the one and two way ANOVA test. As a post test, the Holm Sidak or Tukey tests were applied, with a significance level of 5%. Results: For the microtomographic parameters referring to volume and quality (BV/ Tb.Th, Tb.Sp and Tb.N) the bio material showed improvement in all groups applied with or without PTH 1 34, for BIC it was possible to notice that the ORQ BGPH group was similar to the SHAM BG ( 0.05). For NBA and analysis of fluorochromes, the functionalized biomaterial increased the local bone regeneration of the orchiectomized animal to the same level as the healt hy animal in which biomaterial was used with or without PTH 1 34. Conclusions: The functionalization of BioGran® with PTH 1 34 shows improvement in bone regeneration in animals, being even more effective in animals submitted to osteoporosis. (AU)

• FAPESP's process: 17/08187-3 - Effect of topical PTH, increased to Biogran® on the peri-implant defect in orchiectomized rats
• Grantee: Pedro Henrique Silva Gomes Ferreira
• Support Opportunities: Scholarships in Brazil - Doctorate