Optimazation of cellulase productiion from alternative substrates

Author(s)

Oscar Mauricio Díaz Rodriguez

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química
• Defense date: 2011-08-30

Examining board members

Rubens Maciel Filho; Juliana Teodoro; Patrícia Fazzio Martins Martinez

• Advisor: Rubens Maciel Filho; Célia Maria Araujo Galvão

Abstract

The objective of this work was the optimization of cellulases production, from alternatives substrates, such as sugarcane bagasse, in a process of solid state fermentation by Aspergillus niger. In the first project stage was identified the main components of culture medium: substrate (sugarcane bagasse in natura or pretreated by steam explosion); carbon source (lactose and soybean extract) and the source of nitrogen (peptone and yeast extract). In the second stage of the work, from that variables was optimized cellulases production, according to a central composite rotational design (DCCR). It used a full factorial 2 , including six axial points and 4 replications at the central point. Finally, it using the multiple linear regression was carried out the mathematical models for cellulases production. Thus, we evaluated the influence of selected variables on the production of cellulases quantified as three types of enzymatic activity: total cellulase (FPAse); carboximetilcelulase (CMCase) e p- glucosidase. The maximum production of cellulases was: 0,774 IU/g.d.s of FPAse; 14,41 IU/g.d.s of CMCase and 26,37 IU/g.d.s of p- glucosidase, for a period of 4 days of fermentation. And, the conditions in which maximum production was reached were: concentration of soybean extract 11 g/L, yeast extract concentration of 1,5 g/L and 80% moisture content. These results are very promising, considering the identification of the soybean extract as a inductor of cellulases production, and the least extract, these items are purchased at a low cost in Brazil, that impact in the reduction of global cost of cellulases production (AU)

• FAPESP's process: 09/03534-0 - Optimization of production of cellulases from alternative substrates
• Grantee: Oscar Mauricio Díaz Rodríguez
• Support Opportunities: Scholarships in Brazil - Master