Advanced search
Start date
Betweenand

Molecular cloning, expression, purification and structural characterization of endoglucanase from Trichoderma harzianum aiming the development of enzymatic blends for production of lignocellulosic ethanol

Grant number: 10/18773-8
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): July 01, 2011
Effective date (End): February 29, 2016
Field of knowledge:Biological Sciences - Biophysics
Principal Investigator:Igor Polikarpov
Grantee:Vanessa de Oliveira Arnoldi Pellegrini
Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis, AP.BIOEN.TEM
Associated scholarship(s):13/07884-1 - Study about non-productive binding of cellulolytic enzymes to cellulose and biomass, BE.EP.DD

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BERNARDES, A.; PELLEGRINI, V. O. A.; CURTOLO, F.; CAMILO, C. M.; MELLO, B. L.; JOHNS, M. A.; SCOTT, J. L.; GUIMARAES, F. E. C.; POLIKARPOV, I. Carbohydrate binding modules enhance cellulose enzymatic hydrolysis by increasing access of cellulases to the substrate. Carbohydrate Polymers, v. 211, p. 57-68, MAY 1 2019. Web of Science Citations: 0.
PELLEGRINI, VANESSA O. A.; BERNARDES, AMANDA; REZENDE, CAMILA A.; POLIKARPOV, IGOR. Cellulose fiber size defines efficiency of enzymatic hydrolysis and impacts degree of synergy between endo- and exoglucanases. Cellulose, v. 25, n. 3, p. 1865-1881, MAR 2018. Web of Science Citations: 1.
LIBERATO, MARCELO V.; SILVEIRA, RODRIGO L.; PRATES, ERICA T.; DE ARAUJO, EVANDRO A.; PELLEGRINI, VANESSA O. A.; CAMILO, CESAR M.; KADOWAKI, MARCO A.; NETO, MARIO DE O.; POPOV, ALEXANDER; SKAF, MUNIR S.; POLIKARPOV, IGOR. Molecular characterization of a family 5 glycoside hydrolase suggests an induced-fit enzymatic mechanism. SCIENTIFIC REPORTS, v. 6, APR 1 2016. Web of Science Citations: 9.
PELLEGRINI, VANESSA O. A.; SERPA, VIVIANE ISABEL; GODOY, ANDRE S.; CAMILO, CESAR M.; BERNARDES, AMANDA; REZENDE, CAMILA A.; PEREIRA JUNIOR, NEI; CAIRO, JOAO PAULO L. FRANCO; SQUINA, FABIO M.; POLIKARPOV, IGOR. Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme. Applied Microbiology and Biotechnology, v. 99, n. 22, p. 9591-9604, NOV 2015. Web of Science Citations: 11.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PELLEGRINI, Vanessa de Oliveira Arnoldi. Molecular cloning, expression, purification and structural characterization of endoglucanase from Trichoderma harzianum aiming the development of enzymatic blends for production of lignocellulosic ethanol. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos São Carlos.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.

Filed patent(s) as a result of this research project

OLIGONUCLEOTÍDEOS PARA PRODUÇÃO DE ENDOGLUCANASE, SEQUÊNCIAS PARA PRODUÇÃO DE ENDOGLUCANASE, PROCESSO DE OBTENÇÃO DE ENDOGLUCANASE E USOS DA ENDOGLUCANASE BR1020130108090 - Universidade de São Paulo (USP) . Igor Polikarpov; Vanessa de Oliveira Arnoldi Pellegrini; Viviane Isabel Serpa - May 2013, 02