Molecular cloning, expression, purification and structural characterization of endoglucanase from Trichoderma harzianum aiming the development of enzymatic blends for production of lignocellulosic ethanol

• Grant number: 10/18773-8
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: July 01, 2011
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Igor Polikarpov
• Grantee: Vanessa de Oliveira Arnoldi Pellegrini
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis, AP.BIOEN.TEM
• Associated scholarship(s): 13/07884-1 - Study about non-productive binding of cellulolytic enzymes to cellulose and biomass, BE.EP.DD

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PELLEGRINI, VANESSA O. A.; SERPA, VIVIANE ISABEL; GODOY, ANDRE S.; CAMILO, CESAR M.; BERNARDES, AMANDA; REZENDE, CAMILA A.; PEREIRA JUNIOR, NEI; CAIRO, JOAO PAULO L. FRANCO; SQUINA, FABIO M.; POLIKARPOV, IGOR. Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme. Applied Microbiology and Biotechnology, v. 99, n. 22, p. 9591-9604, NOV 2015. (09/52840-7, 10/18773-8, 08/56255-9, 11/20977-3, 09/05328-9, 11/05712-3)

PELLEGRINI, VANESSA O. A.; BERNARDES, AMANDA; REZENDE, CAMILA A.; POLIKARPOV, IGOR. Cellulose fiber size defines efficiency of enzymatic hydrolysis and impacts degree of synergy between endo- and exoglucanases. Cellulose, v. 25, n. 3, p. 1865-1881, MAR 2018. (12/22802-9, 10/18773-8, 15/13684-0, 16/13602-7)

LIBERATO, MARCELO V.; SILVEIRA, RODRIGO L.; PRATES, ERICA T.; DE ARAUJO, EVANDRO A.; PELLEGRINI, VANESSA O. A.; CAMILO, CESAR M.; KADOWAKI, MARCO A.; NETO, MARIO DE O.; POPOV, ALEXANDER; SKAF, MUNIR S.; et al. Molecular characterization of a family 5 glycoside hydrolase suggests an induced-fit enzymatic mechanism. SCIENTIFIC REPORTS, v. 6, APR 1 2016. (13/08293-7, 09/52840-7, 10/18773-8, 13/15582-5, 08/56255-9, 14/10448-1)

BERNARDES, A.; PELLEGRINI, V. O. A.; CURTOLO, F.; CAMILO, C. M.; MELLO, B. L.; JOHNS, M. A.; SCOTT, J. L.; GUIMARAES, F. E. C.; POLIKARPOV, I.. Carbohydrate binding modules enhance cellulose enzymatic hydrolysis by increasing access of cellulases to the substrate. Carbohydrate Polymers, v. 211, p. 57-68, MAY 1 2019. (10/18773-8, 15/13684-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PELLEGRINI, Vanessa de Oliveira Arnoldi. Molecular cloning, expression, purification and structural characterization of endoglucanase from Trichoderma harzianum aiming the development of enzymatic blends for production of lignocellulosic ethanol. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.