Salivary evaluation in healthy children and in metabolic disorders: clinical and in vitro studies

Author(s)

Maria Carolina Salomé Marquezin

• Document type: Doctoral Thesis
• Press: Piracicaba, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2016-02-25

Examining board members

Paula Midori Castelo; Luciano Jose Pereira; Daniel Araki Ribeiro; Marinês Nobre dos Santos; Renata Cunha Matheus Rodrigues Garcia

• Advisor: Maria Beatriz Duarte Gavião; Paula Midori Castelo

Abstract

Saliva is essential for chewing, speaking, swallowing and oral health. Thus, the aim of this study was to evaluate the salivary characteristics in healthy condition and metabolic changes. The results are presented in three manuscripts. Manuscript 1 shows the evaluation of physicochemical salivary parameters, bite force, masticatory performance and gustatory sensitivity (GS) in 116 prepubertal individuals with normal-weight. Bite force and masticatory performance were evaluated using digital gnatodynamometer and sieving method. Salivary flow rate, pH, total protein (TP), amylase (AMY), calcium (CA) and phosphorus (PHO) concentrations were determined in stimulated (SS) and unstimulated saliva (US) by automated technique. GS was evaluated by identifying the primary tastes (sweet, bitter, salty and sour). Positive correlation between taste sensitivity and age was observed. CA and PHO correlated negatively with salivary flow and pH. Sweet taste scores correlated with AMY concentration; bitter taste sensitivity correlated positively with US flow rate. A weak association between masticatory performance and bite force was observed and there was no significant difference between genders for the parameters evaluated. The concentration of some analytes correlated with saliva flow, pH and gustatory sensitivity. Manuscript 2 presents the study of salivary characteristics and GS (sweet, bitter, salty and sour) of 310 children, 7-10 years old, classified as normal weight, overweight and obese, assessing the association of these parameters with fat percentage. Salivary flow rate and pH were determined in SS and US, as well TP, AMY, CA, urea, triglycerides and PHO. GS and salivary flow did not differ, while SS pH was lower in obese than in overweight. It was observed negative relationship between fat percentage and ? salivary flow (SS-US) and US PHO concentration. Thus, some salivary characteristics may differ among children with different body weights. The manuscript 3 shows the study of inflammatory response of human oral fibroblasts (HGF-1) in contact with artificial saliva (AS I, II and III) an in vitro model. Human saliva was used as control and three AS were treated in HGF-1 in culture medium. The expression of cytokines was assessed by PCR array analysis, Real-Time PCR and immunoassay (using a panel of 12 pro-inflammatory cytokines). After a predefined exposure time of 120 seconds, cells incubated in AS III presented low viability. In PCR array analysis, the expression of inflammatory markers did not differ between treatments; however, a significant reduction in abundance of the protein levels was observed for AS I and AS II compared to control, with a robust anti-inflammatory response to AS II; an effect that may be favorable for patients with hyposalivation and mouth ulcers (AU)

• FAPESP's process: 11/21952-4 - Nutritional, masticatory and salivary evaluation of pre pubertal overweight and obese children
• Grantee: Maria Carolina Salomé Marquezin
• Support Opportunities: Scholarships in Brazil - Doctorate