Ervas, infusões e bebidas prontas-para-consumo à base de ervas: determinação de contaminantes e técnicas de preparo de amostra

Author(s)

Lucas Caldeirão Rodrigues Miranda

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos
• Defense date: 2021-03-19

Examining board members

Helena Teixeira Godoy; Adriana Dillenburg Meinhart; Elza Iouko Ida; Leonardo Fonseca Maciel

• Advisor: Helena Teixeira Godoy; Sara Cristina da Silva Cunha

Abstract

This work aimed to develop, optimize, and validate chromatographic methods intended to determine contaminants in aromatic herbs (n = 20), herbal infusions (n = 20), and ready-do-drink herbal-based beverages (n = 16). This thesis was organized into four chapters. The first one deals with the application of a QuEChERS-LC-MS/MS and an ICP-MS method to determine 14 mycotoxins and 21 toxic elements in herbs and herbal infusions. The second one intended to develop and validate a DLLME-GC-MS method for the determination of 25 pesticides (organochlorine, organophosphorus, and pyrethroid) in herbs and herbal infusions as well. The following chapter deals with the development and the validation of a DLLME-GC-MS/MS method to investigate the presence and content of 5 phthalates and one adipate in herbal-based soft drinks packaged in different packaging materials (polypropylene, polyethylene terephthalate, aluminum, and carton). Finally, the last one handled the development, optimizations, and validation of a LDDES-DLLME-GC-MS/MS to determinate polycyclic aromatic hydrocarbons in packaged herbal-based beverages. The methods showed satisfactory parameters of selectivity, limits of detection, limits of quantification, linearity, precision, and recovery for trace analysis, therefore, they were applied to real samples. Moreover, the method can be considered quick and easy to perform. About the content of contaminants in the samples, only mycotoxins and pesticides showed results above the safe maximum levels (AU)

• FAPESP's process: 17/05048-2 - Plants tadiotionally used for infusion: Sample preparation techniques and determination of contaminants.
• Grantee: Lucas Caldeirão Rodrigues Miranda
• Support Opportunities: Scholarships in Brazil - Doctorate