Development of larvicide from nanoparticles of lignin containing natural actives to combat arbovirosis propagation

Author(s)

Carolina Barbara Rogerio

• Document type: Master's Dissertation
• Press: Bauru. 2023-01-16.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências. Bauru
• Defense date: 2022-12-01
• Advisor: Leonardo Fernandes Fraceto

Abstract

Arboviruses, a critical threat to human health, cause thousands of infections annually and make their control a global public health issue. The prevention of mosquito-borne diseases is strictly linked to the successful management of vector populations. The Aedes aegypti mosquito is known as a vector of arboviruses such as dengue, zika, chinkungunya and yellow fever. Although there are several alternative methods available, vector control is still largely based on the use of synthetic compounds, although useful in combating the vector, it has intensified the selection of mosquitoes resistant to these chemical agents. The use of larvicide is an interesting method for preventing the emergence of the adult mosquito, which, due to its mobility, makes its control more difficult. In this context, several mechanisms are being studied to make vector control more efficient and safer. The development of modified release systems, use of biodegradable products and use of botanical pesticides are examples of methodologies that generate less impact on the environment. The present work presents the development (preparation and characterization) of polymeric nanocapsules with lipid core produced from lignin and containing the oils of neem (Azadirachta indica) and castor oil (Ricinus communis) oils. Allied to good stability, the formulations showed a low toxicity profile in cytotoxicity and genotoxicity assays (HaCaT and Allium Cepa cells), as well as ecotoxicity in non-target organisms (Daphnia magna, Danio rerio, Chironomus riparius and Caenorhabditis elegans), being that nanocapsules loaded with the combination of oils was the formulation that showed greater toxicity in the tests. Larvicidal activity assays in Aedes aegypti larvae, it was possible to determine a lethal concentration of 50% of the larvae only for nanocapsules loaded with neem oil, with LC50 at 3000 mg/L. (AU)

• FAPESP's process: 20/05415-8 - Development of larvicide from lignin nanoparticles containing natural assets to combat the spread of arboviruses
• Grantee: Carolina Barbara Rogerio
• Support Opportunities: Scholarships in Brazil - Master