Full text
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| Author(s): |
Breno Duarte Costa
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) |
| Defense date: | 2022-11-23 |
| Examining board members: |
Bruno Gualano;
Aline David Silva;
Hamilton Augusto Roschel da Silva;
Alessandro Moura Zagatto
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| Advisor: | Bryan Saunders |
| Abstract | |
The aim of this study was to evaluate the acute responses to beta-alanine (BA) supplementation on the plasma concentration of BA, on the expression of genes related to carnosine metabolism and on their respective content in skeletal muscle, before and after chronic BA supplementation. Fourteen healthy and physically active men (participating in weekly physical exercise, but not involved in a structured training program) aged between 18 and 45 years participated. They underwent acute supplementation of 1600 mg of BA before and after a chronic supplementation period of 6400 mg/day for 4 weeks. Muscle samples were collected before, 1-h and 4-h post-acute supplementation, before and after the 4- week supplementation period to assess expression and content of proteins and genes related to carnosine metabolism (CARNS, TAUT, CNDP2, ABAT, PAT1). Acute ingestion of 1600 mg BA up-regulated gene expression (expression >2) of CARNS1, ABAT, TAUT and PAT1 1-hr after supplementation in session 1 (week 0). However, 4 weeks of supplementation of 6400 mg/day BA reduced PAT1 gene expression at 1-hr after acute supplementation) significantly (P=0.04) from baseline. The content of CARNS1 (+246%; P=0.02) and TAUT (+644%; P=0.01) increased acutely 4-h after an acute dose of 1600 mg BA, however after chronic supplementation only TAUT increased its content (+224%) 4-h after the acute dose (P=0.01). We showed that there were acute and chronic changes in gene expression and protein content related to muscle carnosine metabolism. Currently, it is still unclear what this means for muscle carnosine synthesis, however, results of blood BA and muscle carnosine will provide further information depending on how they interact with these findings (AU) | |
| FAPESP's process: | 19/06140-5 - Acute effects of b-alanine intake on regulatory mechanisms of muscle carnosine content |
| Grantee: | Breno Duarte Costa |
| Support Opportunities: | Scholarships in Brazil - Master |