Development of a high-throughput computational system for vigor assessing of soybean seeds

Author(s)

Luiz Gustavo Schultz Senko

• Document type: Master's Dissertation
• Press: Piracicaba.
• Institution: Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC)
• Defense date: 2024-02-08

Examining board members

Francisco Guilhien Gomes Junior; Jose de Barros França Neto; Daniel Teixeira Pinheiro

• Advisor: Francisco Guilhien Gomes Junior

Abstract

The speed and uniformity of seedling emergence in the field are crucial for proper stand establishment and high yields, directly related to seed vigor. In this scenario, computerized image analysis systems emerge as promising alternatives to enhance accuracy and speed in assessing the physiological potential of seeds. The objective of this research was the development of the ProSeedling software, specifically to evaluate the vigor of soybean seeds from seedling images. The acquisition of the seedling images, at three days old, was done by transillumination directly on the germination paper. The study involved two soybean cultivars, NEO610 IPRO and 0590I2X, totaling 18 lots with varied vigor levels. Initially, evaluations were conducted using the Vigor-S software, established as a reference. Subsequently, analyses with ProSeedling provided data for its efficiency validation. The results showed significant correlation coefficients between the ProSeedling and Vigor-S analyses, confirming the accuracy of the new software. In conclusion, ProSeedling proved to be consistent and reliable in evaluating the vigor of soybean seeds, presenting itself as a practical, high-yield, low-cost computerized method, with potential to be widely adopted in the field of seed research and production. (AU)

• FAPESP's process: 21/12321-2 - Development of a high-throughput computational system for vigor assessing of soybean seeds
• Grantee: Luiz Gustavo Schultz Senko
• Support Opportunities: Scholarships in Brazil - Master