Desenvolvimento e aperfeiçoamento de plataformas biotecnológicas baseadas em Saccharomyces cerevisiae

Author(s)

Carla Maneira da Silva

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
• Defense date: 2020-12-21

Examining board members

Gonçalo Amarante Guimarães Pereira; Elizabeth Bilsland; Anderson Ferreira da Cunha

• Advisor: Gonçalo Amarante Guimarães Pereira

Abstract

The ages-long relationship between humans and yeast has reached a new status over the course of the last decades. Swiftly, "Saccharomyces cerevisiae"-based products have shifted from traditional food and beverage goods to a plethora of new economic-relevant products applied in the most diverse sectors. The prosperity of the so-called yeast-based biotechnological platforms is intrinsically linked with the advancements in the genetic engineering field and the advent of metabolic engineering. In this context, the development of the CRISPR/Cas9 system for the efficient and rational genetic engineering of living organisms has completely revolutionized the way we obtain such biotechnological platforms. Together with other CRISPR-based approaches, such as the CRISPR/dCas9 system for genetic expression tuning, these techniques account for a potent genetic toolbox for the creation of suitable S. cerevisiae platforms for the most diverse industrial applications. In this work we address the use of different CRISPR systems for the development and/or improvement of S. cerevisiae-based biotechnological platforms applied in three different highly relevant industries: biofuels, chemicals and diagnostics (AU)

• FAPESP's process: 18/03403-2 - Matabolic engineering of Saccharomyces cerevisiae wild strains using the CRISPR/Cas9 system
• Grantee: Carla Maneira da Silva
• Support Opportunities: Scholarships in Brazil - Master