Influence of photobiomodulation on multipotent mesenchymal cells of adipose tissue in vitro and fibroblasts of patients with sickle cell anemia

Author(s)

Ana Paula Ferro

• Document type: Doctoral Thesis
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC)
• Defense date: 2024-03-26

Examining board members

Elaine Caldeira de Oliveira Guirro; André Capaldo Amaral; Ana Claudia Muniz Renno; José Carlos Tatmatsu Rocha

• Advisor: Elaine Caldeira de Oliveira Guirro; Jayme Adriano Farina Junior

Abstract

Skin wounds on the lower limbs can arise as complications related to sickle cell anemia. Undifferentiated stem cells can differentiate into various cell types to repair injured tissue. Photobiomodulation can promote the proliferation and differentiation of stem cells. The objective of the study is to investigate the effect of different laser and LED photobiomodulation parameters on multipotent mesenchymal stem cells (MSCs), derived from adipose tissue and fibroblasts from individuals with sickle cell anemia, on proliferation and possible nuclear morphological changes in cells mesenchymal trunk. Methods: Mesenchymal stem cells derived from adipose tissue were subjected to low-intensity laser applications (660 nm and 830 nm; with doses of 0.5 J, 2 J and 4 J, powers of 40 mW and 100 mW) and LED (630 nm and 850 nm, with variable frequency from 0 to 1500 Hz and doses of 0.5 J, 2 J and 4 J). After irradiation, the cells were analyzed at 24, 48 and 72 hours with Hoechest staining (viable cells) and Propidium Iodide (dead cells), to analyze the rate of proliferation, death and cell viability. The wound healing assay was performed by scratching the cell layer with a 1000 µL pipette and the analysis was carried out using a confocal microscope and motorized table (LSM 710®, Zeiss, Germany). Both laser and LED wavelengths were effective for cell proliferation, however cell viability with the laser in the first 48 hours after irradiation, the wavelength of 660 nm and power of 100 mW showed significant results, and As time progressed, in 72 hours, the 830 nm laser had a significantly greater increase than the 660 nm laser. With LED, only the 630 nm wavelength had a significant difference. Regarding the healing test, there was a significant reduction in the parameters used compared to the control group. It is concluded that different physical parameters of FBM have biological responses that interfere with the rate of proliferation, death and consequently cell viability. (AU)

• FAPESP's process: 19/09329-1 - Influence of photobiomodulation and electrical stimulation in multipotent mesenchymal cells of adipose tissue in vitro
• Grantee: Ana Paula Ferro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)