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| Author(s): |
Mariana Kolos Galuppo
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-06-29 |
| Examining board members: |
Beatriz Simonsen Stolf Carboni;
Clara Lúcia Barbieri Mestriner;
Mauro Javier Cortez Véliz
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| Advisor: | Beatriz Simonsen Stolf Carboni |
| Abstract | |
Considering the importance of m<font face=\"Symbol\">j in Leishmania´s infection and the potential role of CD100 in the modulation of m<font face=\"Symbol\">j activation, we postulated that this molecule may affect m<font face=\"Symbol\">j infectivity. Therefore, we analyzed and compared the expression of CD100 on different types of m<font face=\"Symbol\">j (BALB/c and C57BL/6) and their effects in terms of m<font face=\"Symbol\">j activation and infectivity by L. amazonensis. We analyzed the expression of CD100 and the results suggest that m<font face=\"Symbol\">j from BALB/c express more CD100 than C57BL/6 and this expression decrease along the infection. M<font face=\"Symbol\">j from BALB/c pre-treated with sCD100 have augmented infection rates in 4h and in contact with the sCD100 before, during and after infection, increase rates in periods of 4 and 24h. In m<font face=\"Symbol\">j from C57BL/6 pre-treated with sCD100 or infection period, the rates increase in 4h. When macrophages remained in contact with sCD100 throughout the infection, rates increase after 4 and 24h. We can therefore conclude that CD100 may be related to modulation of Leishmania´s infection and that the infection itself affects CD100 expression in m<font face=\"Symbol\">j. (AU) | |
| FAPESP's process: | 10/02235-7 - Analysis of CD100 activity in the modulation of macrophage activation and infection by Leishmania (Leishmania) amazonensis |
| Grantee: | Mariana Kolos Galuppo |
| Support Opportunities: | Scholarships in Brazil - Master |