Extraction of hemicellulose from sugarcane bagasse for xylooligosaccharides production

Author(s)

Michel Brienzo

• Document type: Doctoral Thesis
• Press: Lorena.
• Institution: Universidade de São Paulo (USP). Escola de Engenharia de Lorena (EEL/ASDI)
• Defense date: 2010-03-26

Examining board members

Adriane Maria Ferreira Milagres; Luis Henrique Souza Guimarães; Nathália Ramos de Melo; George Jackson de Moraes Rocha; Roberto da Silva

• Advisor: Adriane Maria Ferreira Milagres

Abstract

Hemicellulose extracted from sugarcane bagasse was hydrolyzed by enzymes from Thermoascus aurantiacus, Trichoderma reesei and Aspergilus niger to cause the degradation of xylan to xylooligosaccharides (XOs). Hemicellulose was extracted with hydrogen peroxide in the presence of antraquinone, sodium sulphite or hydrogen peroxide. Hemicelluloses extracted with antraquinone or sulphite presented low solubility in water, which is not appropriated to enzymatic hydrolysis. To maximize the hemicellulose yields several extraction conditions were examined applying the 24 factorial design: H2O2 concentration from 2 to 6% (w/v), reaction time from 4 to 16 h, temperature from 20 to 60°C, and magnesium sulfate absence or presence (0.5%, w/v). This approach allowed selection of conditions for the extraction of low and high lignin content hemicellulose. At midpoint the yield of hemicellulose was 94.5% with more than 88% of lignin removed. Hemicellulose in 86% yield with low lignin content (5.9%) was obtained with 6% H2O2 treatment for 4 h and 20°C. This hemicellulose is much lighter in color than samples obtained at the midpoint condition and was found suitable for subsequent enzymatic hydrolysis. The molecular weight of hemicellulose was 21,000 g/mol with composition of aproximately 81% xylose, 4% arabinose, 4% glucose and 3% uronic acids, high water solubility (90 %). Enzymes for hemicellulose hydrolysis were produced by the fungi on wheat bran. Cellulases and hemicellulases were present in all extracts especially the endo-β-1,4-xylanase. The profile of production of XOs obtained on hydrolysis with enzymes from T. aurantiacus and T. reesei was similar, with the main product xylobiose, followed by xylose, xylotriose, xylotetraose and xylopentaose, and these XOs showed linear chain. The hydrolysis of hemicellulose with enzymes of A. niger produced exclusively xylose, a consequence of β-xylosidase content. The rate of conversion of hemicellulose in XOs with enzymes of T. reesei was higher at the beginning of the reaction (6 h), decreasing from 24 h, when starts the production of xylose. The influence of substrate concentration and loading of xylanase in conversion of hemicellulose to XOs was evaluated by an 22 full factorial design with centered face. Optimization of hydrolysis (2.6% substrate and 60 U/g endo-β-1,4-xylanase) with the extract of T. aurantiacus resulted in 42 % conversion XOs. The optimization with the extract of T. reesei resulted in a conversion of hemicellulose up to 20%, with optimal substrate 3.8% and 87.5 U/g endo-β-1,4-xylanase. The efficiency of hydrolysis by enzymes from T. aurantiacus was superior to commercial extracts, and showed ability to degrade hemicelluloses of different compositions (birchwood and oat spelt). The structural differences, such as branches and lignin content did not affect the action of these enzymes. The differences in the efficiency and extent of enzymatic hydrolysis by enzymes of these fungi might have occurred in function of differences in physicochemical properties and specific activity. The enzymatic hydrolysis was more appropriate for production of XOs than autohydrolysis, which generated predominantly xylose and formation of furfural. Despite of short reaction time, the production of XOs was low and purification is needed in order to obtain a final product with desirable characteristics. (AU)

• FAPESP's process: 06/03564-9 - Extraction of hemicellulose from sugarcane bagasse to obtain pentoses and xylooligossacharides
• Grantee: Michel Brienzo
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)