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| Author(s): |
Eduardo Bertoli Belai
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Bauru. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) |
| Defense date: | 2011-08-25 |
| Examining board members: |
Ana Paula Campanelli;
Gustavo Pompermaier Garlet;
Thais Helena Gasparoto
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| Advisor: | Ana Paula Campanelli |
| Abstract | |
Modulation of immune response in tumor site is a critical mechanism involved with tumor evasion. PD-1 and PD-L1/PD-L2 signals could be involved in tumor escape. However, little is known about the role of these molecules in squamous cell carcinoma development. In the present study, we investigated the expression of PD-1 on T cells from squamous cell carcinoma lesions and lymph nodes. Skin carcinogenesis was chemically induced with DMBA/PMA in mice. Characterization of PD-1, PD-L1 and PD-L2 expression in the lesion and lymph nodes were analysed by flow cytometry. IL-10, IL-12, TGF- and IFN- production was determined by ELISA. DMBA/PMA-treated mice showed higher rate of papillomas and progression to squamous cell carcinoma. We also found that CD4+PD-1+ T cells migrated to the tumor site and that higher level of CD4+PD-1+, CD8+PD-1+, CD14+PD-1+ and CD4+PD-L1+ were detected in the lymph nodes when compared with the control group. Besides, IL-12, IFN- and TGF-, but not IL-10, productions were higher detected in the lesions compared with the control group. These data indicate that PD-1 expression is up-regulated on tumor infiltrating lymphocytes and lymph nodes. This fact and high levels of TGF- and IL-10 may contribute to suppression of anti-tumor immune response. (AU) | |
| FAPESP's process: | 09/03471-9 - Modulation of immune response during squamous-cell carcinoma development and progression |
| Grantee: | Eduardo Bertoli Belai |
| Support Opportunities: | Scholarships in Brazil - Master |