Geraldo Aleixo da Silva Passos Júnior

• Abstract

  The Autoimmune regulator (Aire) gene located on the human chromosome 21q22.3 or on mouse (Mus musculus) 10qC1, is a controller of the central immune tolerance and consequently the onset of autoimmune diseases. Aire share ~ 80% human-mouse similarity and it regulates the transcription of genes that encode peripheral tissue antigens (PTAs) in medullary thymic epithelial cells (mTECs) (promi…

  Effect of CRISPR-Cas9-induced mutations in the Aire gene (APS1 syndrome) on protein conformation, mTEC cell transcriptome and their interaction with thymocytes, AP.TEM

• Abstract

  The thymus plays a role in the induction of immunological tolerance to tissue related antigens (TRA). Within the thymic medulla, the medullary thymic epithelial cells (mTECs) express hundreds of these TRAs, which, in turn, represent virtually all organs and tissues of the body. Due to the diversity of representation, this phenomenon was called promiscuous gene expression (PGE) which is la…

  The function of the AIRE gene and of Micro RNAs in the control of adhesion between medullary thymic epithelial cells with thymocytes, AP.R

• Abstract

  Molecular genetic studies will be performed on type-1 (T1-D), type-2 (T2-D) and gestational (GD) diabetes, evaluating the large scale gene expression profiles (transcriptome level). T1-D is an autoimmune disorder caused by the immunological destruction of pancreatic β- cells, leading to insulin dependence. Although several susceptibility loci have been described in association with T1-D,…

  Control of the transcriptome in diabetes mellitus, AP.TEM

(Only some records are available in English at this moment)

• Effect of Crispr-Cas9-induced mutations in the Aire gene on transcriptional, post-transcriptional activity of mTEC cells and their function on thymocyte migration, BP.TT

• Abstract

  The post-doc fellow will study the effect of mutations in the Aire gene, associated with the APS1 syndrome, on two of the essential processes of mTEC cells during the induction of central immunological tolerance in the thymus, that is, the thymocyte migration - mTECs, which is necessary for adhesion to occur between these cells. Also, we will assess whether these mutations influence the t…

  Effect of aire gene mutations induced by CRISPR-Cas9 on the transcriptional and post-transcriptional activity of mTEC cells and in its function on the thymocyte migration, BP.PD

• Abstract

  The fellow will study the transcriptional profile involving mRNAs and miRNAs of thymocytes that physically interact with Aire wild-type (wt) or Aire mutant mTECs cells (adhesion mTECs-thymocytes in vitro). As discussed in the thematic project, the interaction between thymocytes and epithelial thymic cells (TECs) is essential for the development of these two cell types. To test this hypoth…

  Transcriptional and post-transcriptional activity of thymocytes adhered to Aire mutant mTEC cells, BP.DD

(Only some records are available in English at this moment)