MMP-9 Expression Increases According to the Grade of Squamous Intraepithelial Lesion in Cervical Smears

Author(s)

Matheus, Erika R. ^[1] ; Zonta, Marco A. ^[2] ; Discacciati, Michelle G. ^[1] ; Paruci, Priscila ^[2] ; Velame, Fernanda ^[2] ; Cardeal, Laura B. S. ^[1] ; Barros, Silvia B. M. ^[1] ; Pignatari, Antonio C. ^[3] ; Maria-Engler, Silvya S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin Chem & Toxicol, Lab Clin Pathol & Cytol, BR-05508000 Sao Paulo - Brazil

^[2] In Cito Lab Diagnost Cytol, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Med, Special Lab Clin Microbiol, BR-05508000 Sao Paulo - Brazil

• Document type: Journal article
• Source: DIAGNOSTIC CYTOPATHOLOGY; v. 42, n. 10, p. 827-833, OCT 2014.
• Web of Science Citations: 5

Abstract

Studies about cervical carcinogenesis have demonstrated the increased expression of matrix-metalloproteinase (MMP) according to the grade of cervical intraepithelial lesions. Considering the importance of innovative techniques to introduce noninvasive and rapid diagnoses for patients, this study aimed to perform MMP-9 immunocytochemistry in cervical smears according to the cytopathological diagnoses, in order to monitor MMP activity in cervical smears. This cross-sectional study investigated the expression of MMP-9 in normal cervical smears, inflammatory cervical smears, squamous intraepithelial lesions, and cervical carcinoma. Cervical smears from 630 women were collected for cytopathological diagnoses and immunocytochemistry. Women with squamous intraepithelial lesions showed an increase in MMP-9 expression, with moderate to intense staining occurring with increasing cervical lesion grade. The prevalence of moderate to intense MMP-9 staining was 9% in normal cervical smears, 12% in cervical inflammation, 24% in low-grade squamous intraepithelial lesion (LSIL), 92% in high-grade squamous intraepithelial lesions (HSIL) and 100% in cervical carcinoma cases. In the specific case of LSIL, we found that association with MMP-9 is more evident when there is the simultaneous presence of an infectious agent. Thus, the expression of MMP-9 in cervical smears increases according to the grade of cervical lesion and LSIL in the presence of infectious agents showed higher MMP-9 expression than women with LSIL without infectious agents. Diagn. Cytopathol. 2014;42:827-833. (c) 2014 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 08/58817-4 - Generation of human artificial skins and invasive melanomas as a platform for pharmacological testing
• Grantee: Silvya Stuchi Maria-Engler
• Support type: Regular Research Grants
• FAPESP's process: 12/09746-2 - Relationship between MMP-9 and RECK expression in abnormal cervical smears and clinical evolution of squamous intraepithelial lesion
• Grantee: Michelle Garcia Discacciati de Carvalho
• Support type: Scholarships in Brazil - Post-Doctorate