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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation and characterization of microsatellite loci for Bixa orellana, an important source of natural dyes

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Author(s):
Dequigiovanni, G. [1] ; Ramos, S. L. F. [1] ; Zucchi, M. I. [2] ; Bajay, M. M. [1] ; Pinheiro, J. B. [1] ; Fabri, E. G. [3] ; Bressan, E. A. [1] ; Veasey, E. A. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Piracicaba, SP - Brazil
[2] Agencia Paulista Tecnol Agronegocios, Polo Apta Ctr Sul, Piracicaba, SP - Brazil
[3] Inst Agron Estado Sao Paulo, Ctr Hort, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Genetics and Molecular Research; v. 13, n. 4, p. 9097-9102, 2014.
Web of Science Citations: 6
Abstract

Annatto (Bixa orellana) is a plant native from the American continental tropical zone. The seeds are used to produce a carotenoid-based yellow to orange food coloring. Microsatellite markers were developed for the Brazilian native species Bixa orellana to describe its genetic diversity and structure as well as to support conservation studies. Twenty-five microsatellite loci were isolated and characterized using an enriched genomic library. Ten loci were polymorphic in the 50 accessions sampled in this study, while 15 were considered monomorphic. The mean number of alleles per locus was 3.8, ranging from 2 to 6 alleles per locus. Mean values for the observed and expected heterozygosities were 0.541 (ranging from 0 to 0.658) and 0.639 (ranging from 0.422 to 0.787), respectively. All markers described in this study will be useful in further studies evaluating the genetic diversity, population dynamics, and conservation genetics of Bixa orellana. (AU)

FAPESP's process: 13/08884-5 - Genetic structure and mating system of annatto (Bixa orellana L.) populations from the Brazilian Amazon and Central Brazil using microsatellite markers
Grantee:Gabriel Dequigiovanni
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/08307-5 - Genetic structure and mating system of local varieties and wild populations of annatto (Bixa orellana L.) in Brazilian Amazonia and Central Brazil using microsatellite markers
Grantee:Elizabeth Ann Veasey
Support type: Regular Research Grants
FAPESP's process: 11/05243-3 - Development of microsatellite loci for genetic diversity characterization of annatto (Bixa orellana) accessions
Grantee:Gabriel Dequigiovanni
Support type: Scholarships in Brazil - Master