Isolation and characterization of microsatellites markers for two South American frogs (Leptodactylus bufonius and L. chaquensis) using next generation sequencing

Author(s)

Faggioni, Gabriel P. ^[1] ; Zamudio, Kelly R. ^[2] ; Souza, Franco L. ^[1] ; Prado, Cynthia P. A. ^[3]

Affiliation

^[1] Univ Fed Mato Grosso do Sul, Programa Posgrad Ecol & Conservacao, BR-79070900 Campo Grande, MS - Brazil

^[2] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 - USA

^[3] Univ Estadual Paulista, Dept Morfol & Fisiol Anim, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP - Brazil

• Document type: Journal article
• Source: Amphibia-Reptilia; v. 35, n. 4, p. 405-412, 2014.
• Web of Science Citations: 1

Abstract

Leptodactylus bufonius (Vizcacheras' white-lipped frog) and L. chaquensis (Cei's white-lipped frog) are pond-breeding frogs that inhabit the Chaco and surrounding savanna-like formations in South America. Throughout the Chacoan plain, the combined impacts of livestock and forestry practices have led to a highly fragmented landscape and an impoverished ecological system, threatening local species. We cloned and characterized new microsatellite markers for both species. These markers will be useful for behavioral and conservation genetic studies of populations throughout this threatened habitat. (AU)

• FAPESP's process: 09/12013-4 - Reproductive ecology of anuran amphibians: an evolutionary perspective
• Grantee: Cynthia Peralta de Almeida Prado
• Support Opportunities: Research Grants - Young Investigators Grants