Arterial tissue and plasma concentration of enzymatic-driven oxysterols are associated with severe peripheral atherosclerotic disease and systemic infl ammatory activity

Author(s):Show less -

Virginio, V. W. M. ^{[1, 2, 3]} ; Nunes, V. S. ^[4] ; Moura, F. A. ^[1] ; Menezes, F. H. ^[5] ; Andreollo, N. A. ^[5] ; Rogerio, F. ^[6] ; Scherrer, D. Z. ^{[2, 3]} ; Quintao, E. C. R. ^[4] ; Nakandakare, E. ^[4] ; Petrucci, O. ^[5] ; Nadruz-Junior, W. ^[1] ; de Faria, E. C. ^{[2, 3]} ; Sposito, A. C.

Affiliation

^[1] Univ Estadual Campinas, Fac Med Sci, Dept Cardiol, UNICAMP, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Med Sci, Lipid Lab, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Med Sci, Ctr Med & Expt Surg, Campinas, SP - Brazil

^[4] Univ Sao Paulo, Fac Med Sci, Lipids Lab LIM 10, Sao Paulo - Brazil

^[5] Univ Estadual Campinas, Fac Med Sci, UNICAMP, Dept Surg, Campinas, SP - Brazil

^[6] Univ Estadual Campinas, Fac Med Sci, UNICAMP, Dept Pathol, Campinas, SP - Brazil

• Document type: Journal article
• Source: Free Radical Research; v. 49, n. 2, p. 199-203, FEB 2015.
• Web of Science Citations: 14

Abstract

Introduction. Cholesterol undergoes oxidation via both enzymatic stress-and free radical-mediated mechanisms, generating a wide range of oxysterols. In contrast to oxidative stress-driven metabolites, enzymatic stress-derived oxysterols are scarcely studied in their association with atherosclerotic disease in humans. Methods. 24S-hydroxycholesterol (24S-HC), 25-hydroxycholesterol (25-HC), and 27-hydroxycholesterol (27-HC) were assessed in plasma and arteries with atherosclerotic plaques from 10 patients (54 -84 years) with severe peripheral artery disease (PAD) as well as arteries free of atherosclerotic plaques from 13 individuals (45 - 78 years, controls). Results. Plasma 25-HC was higher in PAD individuals than in controls (6.3{[}2] vs. 3.9{[}1.9] ng/mgCol; p = 0.004). 24S-HC and 27-HC levels were, respectively, five-and 20-fold higher in the arterial tissue of PAD individuals than in those of the controls (p = 0.016 and p = 0.001). Plasma C-reactive protein correlated with plasma 24-HC (r = 0.51; p = 0.010), 25-HC (r = 0.75; p < 0.001), 27-HC (r = 0.48; p = 0.015), and with tissue 24S-HC (r = 0.4; p = 0.041) and 27-HC (r = 0.46; p = 0.023). Conclusion. Arterial intima accumulation of 27-HC and 24S-HC is associated with advanced atherosclerotic disease and systemic inflammatory activity in individuals with severe PAD. (AU)

• FAPESP's process: 06/60585-9 - Relation of the plasma HDL-cholesterol concentration with blood monocyte and whole body cholesterol metabolism parameters
• Grantee: Eder Carlos Rocha Quintão
• Support Opportunities: Research Projects - Thematic Grants