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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bacterial killing via a type IV secretion system

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Author(s):
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Souza, Diorge P. [1] ; Oka, Gabriel U. [1] ; Alvarez-Martinez, Cristina E. [1, 2] ; Bisson-Filho, Alexandre W. [1] ; Dunger, German ; Hobeika, Lise [1] ; Cavalcante, Nayara S. [3] ; Alegria, Marcos C. [1] ; Barbosa, Leandro R. S. [4] ; Salinas, Roberto K. [1] ; Guzzo, Cristiane R. [1, 5] ; Farah, Chuck S. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, BR-13083862 Campinas, SP - Brazil
[3] Univ Sao Paulo, Inst Fis Sao Carlos, Dept Fis & Informat, BR-13566590 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Inst Fis, Dept Fis Geral, BR-05315970 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: NATURE COMMUNICATIONS; v. 6, MAR 2015.
Web of Science Citations: 40
Abstract

Type IV secretion systems (T4SSs) are multiprotein complexes that transport effector proteins and protein-DNA complexes through bacterial membranes to the extracellular milieu or directly into the cytoplasm of other cells. Many bacteria of the family Xanthomonadaceae, which occupy diverse environmental niches, carry a T4SS with unknown function but with several characteristics that distinguishes it from other T4SSs. Here we show that the Xanthomonas citri T4SS provides these cells the capacity to kill other Gram-negative bacterial species in a contact-dependent manner. The secretion of one type IV bacterial effector protein is shown to require a conserved C-terminal domain and its bacteriolytic activity is neutralized by a cognate immunity protein whose 3D structure is similar to peptidoglycan hydrolase inhibitors. This is the first demonstration of the involvement of a T4SS in bacterial killing and points to this special class of T4SS as a mediator of both antagonistic and cooperative interbacterial interactions. (AU)

FAPESP's process: 05/59243-3 - Structural and functional analysis of multi-prosthetic systems for the pathogenicity of Xanthomonas axonopodis pv. citri
Grantee:Shaker Chuck Farah
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 09/54009-3 - Acquisition of a 700 MHz nuclear magnetic resonance instrument with cryogenically cooled probe for the Analytical Chemistry Center of the University of São Paulo
Grantee:Shaker Chuck Farah
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 11/07777-5 - Cyclic di-GMP signaling and the Type IV macromolecule secretion system in Xanthomonas citri
Grantee:Shaker Chuck Farah
Support Opportunities: Research Projects - Thematic Grants