Cloning, Characterization and Anti-Inflammatory Properties of Bothrops jararaca Snake Antithrombin

Author(s)

de Morais-Zani, Karen ^{[1, 2]} ; Grego, Kathleen F. ^[1] ; Torquato, Ricardo J. S. ^[3] ; Silva, Caroline S. ^[1] ; Tanaka, Aparecida S. ^[3] ; Tanaka-Azevedo, Anita M. ^{[1, 2]}

Affiliation

^[1] Inst Butantan, Lab Herpetol, BR-05503900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Butantan, Inst Pesquisas Tecnol, Programa Posgrad Interunidades Biotecnol, BR-05508900 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo - Brazil

• Document type: Journal article
• Source: PROTEIN AND PEPTIDE LETTERS; v. 22, n. 5, p. 410-418, 2015.
• Web of Science Citations: 1

Abstract

Antithrombin inhibits blood coagulation through the interaction with serine proteases in both intrinsic and extrinsic pathways. In addition, antithrombin also shows anti-inflammatory properties, which are independent of its effects on coagulation. This work shows for the first time the cloning and sequencing of antithrombin from a snake species. This predicted protein is composed by 430 amino acids and presents about 64.5% sequence identity to human antithrombin. Biacore experiments revealed that the binding affinity of Bothrops jararaca snake antithrombin to heparin was similar to 30 times higher than that of human antithrombin. Furthermore, Bothrops jararaca antithrombin is more effective in preventing acute inflammation induced by carrageenan when compared to human antithrombin. Hence, the results showed herein suggest that Bothrops jararaca antithrombin can play a key role in the control of acute inflammation and that this molecule might be used as a pharmacological tool and as a prototype for drug development. (AU)

• FAPESP's process: 09/53844-6 - Installation of a Surface Plasmon Resonance System at the Federal University of São Paulo
• Grantee: Aparecida Sadae Tanaka
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 11/21558-4 - Study of anti-inflammatory activity of Bothropoides jararaca antithrombin by proteomic analysis
• Grantee: Karen de Morais Zani
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 09/03484-3 - Study of anti-inflammatory activity of antithrombin of Bothrops jararaca snake: antithrombin cloning and expression
• Grantee: Anita Mitico Tanaka-Azevedo
• Support Opportunities: Regular Research Grants