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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cloning, Characterization and Anti-Inflammatory Properties of Bothrops jararaca Snake Antithrombin

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Author(s):
de Morais-Zani, Karen [1, 2] ; Grego, Kathleen F. [1] ; Torquato, Ricardo J. S. [3] ; Silva, Caroline S. [1] ; Tanaka, Aparecida S. [3] ; Tanaka-Azevedo, Anita M. [1, 2]
Total Authors: 6
Affiliation:
[1] Inst Butantan, Lab Herpetol, BR-05503900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Butantan, Inst Pesquisas Tecnol, Programa Posgrad Interunidades Biotecnol, BR-05508900 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PROTEIN AND PEPTIDE LETTERS; v. 22, n. 5, p. 410-418, 2015.
Web of Science Citations: 1
Abstract

Antithrombin inhibits blood coagulation through the interaction with serine proteases in both intrinsic and extrinsic pathways. In addition, antithrombin also shows anti-inflammatory properties, which are independent of its effects on coagulation. This work shows for the first time the cloning and sequencing of antithrombin from a snake species. This predicted protein is composed by 430 amino acids and presents about 64.5% sequence identity to human antithrombin. Biacore experiments revealed that the binding affinity of Bothrops jararaca snake antithrombin to heparin was similar to 30 times higher than that of human antithrombin. Furthermore, Bothrops jararaca antithrombin is more effective in preventing acute inflammation induced by carrageenan when compared to human antithrombin. Hence, the results showed herein suggest that Bothrops jararaca antithrombin can play a key role in the control of acute inflammation and that this molecule might be used as a pharmacological tool and as a prototype for drug development. (AU)

FAPESP's process: 09/53844-6 - Installation of a Surface Plasmon Resonance System at the Federal University of São Paulo
Grantee:Aparecida Sadae Tanaka
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 11/21558-4 - Study of anti-inflammatory activity of Bothropoides jararaca antithrombin by proteomic analysis
Grantee:Karen de Morais Zani
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 09/03484-3 - Study of anti-inflammatory activity of antithrombin of Bothrops jararaca snake: antithrombin cloning and expression
Grantee:Anita Mitico Tanaka-Azevedo
Support Opportunities: Regular Research Grants