|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||September 01, 2012|
|Effective date (End):||August 31, 2013|
|Field of knowledge:||Biological Sciences - Physiology - Compared Physiology|
|Principal Investigator:||Anita Mitico Tanaka-Azevedo|
|Grantee:||Daniela Miki Hatakeyama|
|Home Institution:||Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil|
Belonging to the family Viperidae, the genus Bothrops has more than 30 species and subspecies of snakes distributed between Central America and South America. This genus is important from a clinical standpoint, accounting for around 70% of snakebites in Brazil.The study of snake venoms is an important area of biomedical research, mainly because of their pathophysiological actions, such as neurotoxic, hemorrhagic, coagulant and tissue damage.Regarding the Bothrops venom, three pathophysiologic activities are described : proteolytic, or acute inflammatory, coagulant and hemorrhagic.The elucidation of these activities is important both for research related to poisons, and for the production of sera antiophidic, considering that, if present, the variations can result in the production of sera less effective and inconsistent results in scientific research. In this context, although there is a search for standardization and stabilization of the different components of snake venoms, still lack conclusive results on both aspects.There is a clear need for the use of poisons references to national as well as the standardization of testing methods of the biological activities of snake venoms in Brazil to ensure uniformity in ophidian serums. Thus, the Butantan Institute became responsible for the preparation and distribution of poisons, both for the preparation of ophidian serums, such as for use in research.In this context, considering the importance of obtaining poisons whose properties are preserved and well determined, and the lack of information about the stability of poisons with respect to collection time, we intend to study the longevity of biological activities and biochemical characteristics of the batches of venoms Bothrops jararaca extracted in the Herpetology Laboratory of the Butantan Institute from 1960 until 2008.