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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

beta-Lactamase-based biosensor for the electrochemical determination of benzylpenicillin in milk

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Author(s):
do Prado, Thiago M. [1] ; Foguel, Marcos V. [1] ; Goncalves, Luis Moreira [2] ; Sotomayor, Maria del Pilar T. [1]
Total Authors: 4
Affiliation:
[1] IQ UNESP, Araraquara, SP - Brazil
[2] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, P-4169007 Oporto - Portugal
Total Affiliations: 2
Document type: Journal article
Source: SENSORS AND ACTUATORS B-CHEMICAL; v. 210, p. 254-258, APR 2015.
Web of Science Citations: 12
Abstract

Antibiotics are widely used in dairy cattle production, however this can pose a public health threat, particularly when antibiotic residues remain in the food products like milk. Therefore, new analytical solutions aiming the determination of antibiotics, like the still commonly used penicillin, are welcomed. This work describes a novel electrochemical biosensor, with amperometric and differential pulse voltammetric detection, based on a modified carbon paste with beta-lactamase for the determination of penicillin G, a beta-lactam antibiotic. It is a `second-generation' biosensor since cobalt phthalocyanine was used as an electron mediator. The proposed biosensor, by differential pulse voltammetry, presented a limit of detection of 0.079 mu mol/L. Using this biosensor, a value of 43 +/- 3 mu mol/L was determined for the Michaelis-Menten enzymatic constant for penicillinase extracted from Bacillus cereus. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/17552-0 - Detection and quantification of environmental important dyes using biomimetic sensors based on fiber optics and molecularly imprinted polymers
Grantee:Marcos Vinicius Foguel
Support type: Scholarships in Brazil - Doctorate