Determination of the phenolic composition from Brazilian tropical fruits by UHPLC-MS/MS

Author(s)

Bataglion, Giovana A. ^[1] ; da Silva, Felipe M. A. ^[2] ; Eberlin, Marcos N. ^[1] ; Koolen, Hector H. F. ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, UNICAMP, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Univ Fed Amazonas, Dept Chem, UFAM, BR-69077000 Manaus, AM - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 180, p. 280-287, AUG 1 2015.
• Web of Science Citations: 49

Abstract

Although Brazil is the third largest fruit producer in the world, several specimens consumed are not well studied from the chemical viewpoint, especially for quantitative analysis. For this reason and the crescent employment of mass spectrometry (MS) techniques in food science we selected twenty-two phenolic compounds with important biological activities and developed an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method using electrospray (ESI) in negative ion mode aiming their quantification in largely consumed Brazilian fruits (acai-do-Amazonas, acerola, cashew apple, camu-camu, pineapple and tapereba). Multiple reaction monitoring (MRM) was applied and the selection of proper product ions for each transition assured high selectivity. Linearity (0.995 < r(2) < 0.999), limit of detection (28.85-3333 pg/mL), limit of quantification (96.15-1111 pg/mL), inter- and intraday accuracy (>80%), precision (CV < 20%) and extraction recovery rate (>80%) were satisfactory and showed that the method provides an efficient protocol to analyze phenolic compounds in fruit pulp extracts. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 12/21395-0 - Molecular characterization of sedimentary organic matter in the Estuary-Lagoon System Cananéia-Iguape by Fourier transform ion cyclotron resonance mass spectrometry
• Grantee: Giovana Anceski Bataglion
• Support Opportunities: Scholarships in Brazil - Doctorate