Potential of a bacterial consortium to degrade azo dye Disperse Red 1 in a pilot scale anaerobic-aerobic reactor

Author(s)

Franciscon, Elisangela ^[1] ; Mendonca, Danilo ^[1] ; Seber, Samile ^[1] ; Morales, Daniel Alexandre ^[1] ; Zocolo, Guilherme Juliao ^[2] ; Zanoni, MariaValnice Boldrin ^[2] ; Grossman, Matthew James ^[3] ; Durrant, Lucia Regina ^[3] ; Freeman, Harold S. ^[4] ; Umbuzeiro, Gisela Aragao ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Technol, BR-13484332 Limeira, SP - Brazil

^[2] Paulista Julio Mesquita Filho State Univ, Inst Chem, BR-14801970 Araraquara, SP - Brazil

^[3] Univ Estadual Campinas, Food Engn Fac, BR-13083970 Campinas, SP - Brazil

^[4] N Carolina State Univ, Dept Text Engn Chem & Sci, Raleigh, NC 27695 - USA

• Document type: Journal article
• Source: Process Biochemistry; v. 50, n. 5, p. 816-825, MAY 2015.
• Web of Science Citations: 9

Abstract

The textile industry is the largest consumer of azo dyes and activated sludge treatment systems typically used to remove them are frequently inefficient, resulting in significant quantities of these compounds adsorbed in the sludge or released into water bodies. The aim of this study was to evaluate the biodegradation of the commercial azo dye Disperse Red 1 in a pilot scale anaerobic-aerobic reactor inoculated by a microbial consortium selected for its capacity to degrade azo dyes. Dye decolorization (80%) occurred in 60 h in the anaerobic reactor and 92% COD (chemical oxygen demand) was removed as measured in the treated effluent. LC-ESI-MS/MS of the treated effluent showed the formation of a reduced azo bond and azo bond cleavage and subsequent degradation products. Aromatic amines produced by the cleavage of the azo bond were not detected. Toxicity tests performed with Daphnia similis and Hydra attenuate in the treated effluent showed that the toxicity was reduced significantly. Mutagenicity, evaluated using the Salmonella/microsome Ames test, found the untreated dye medium was only mutagenic toward Salmonella strain YG1041. However after treatment, only marginal mutagenicity was observed. These results indicate the bacterial consortium was effective in biodegrading Disperse Red 1 and also reducing the toxicity and mutagenicity and may represent a promising application for dyes biodegradation. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 09/12739-5 - Eco/genotoxicity of commercial dye CI Disperse Red 1 and chlorinated byproducts.
• Grantee: Francine Inforçato Vacchi
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 08/10449-7 - Assessment of occurrence, toxicity/genotoxicity and degradation processes of dyes in effluents and surface water
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants