Effect of super short pulse Er:YAG laser on human dentinScanning electron microscopy analysis

Author(s)

Trevelin, Livia Tosi ^[1] ; Marques, Marcia Martins ^[1] ; Correa Aranha, Ana Cecilia ^[1] ; Arana-Chavez, Victor Elias ^[2] ; Matos, Adriana Bona ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Biomat & Oral Biol, BR-05508900 Sao Paulo - Brazil

• Document type: Journal article
• Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 78, n. 6, p. 472-478, JUN 2015.
• Web of Science Citations: 6

Abstract

This study evaluated the effect of different pulse widths in the morphological characteristics of human dentin irradiated with Er:YAG in cavity preparation protocols and dentin pretreatment. Dentin discs with 2 mm thickness were obtained from 18 human molars. The experimental groups were composed from two variables: (1) clinical protocolcavity preparation (E=200 mJ/20 Hz)and pretreatment (E=80 mJ/2 Hz); and (2) pulse duration50, 300, and 600 s. This formed six experimental groups (n=3): G1 (E=200 mJ/20 Hz/50 s); G2 (E=200 mJ/20 Hz/300 s); G3 (E=200 mJ/20 Hz/600 s); G4 (E=80 mJ/2 Hz/50 s); G5 (E=80 mJ/2 Hz/300 s); G6 (E=80 mJ/2 Hz/600 s). The samples were irradiated with the Er:YAG laser by noncontact mode at a focal distance of 7 mm from the target point under continuous water spray (60% water and 40% air). After the irradiation, they were processed for scanning electron microscopy (SEM). Morphological analysis showed an irregular dentin surface, absence of smear layer with opening of the exposure of dentinal tubules and protruding peritubular dentinwithout indications of changes for all protocols used. Regardless of the analyzed experimental group, the dentin surface showed a microretentive morphology characteristic of ablation. The G1 and G4 showed a rougher surface when compared to other groups. Finally, we concluded that the pulse width can influence the morphological characteristics of the irradiated dentin tested in different clinical indications. The larger surface irregularity caused by regulation with less pulse width (50 mu s) seems more appropriate to get a microretentive pattern necessary for successful adhesives restoration procedures. Microsc. Res. Tech. 78:472-478, 2015. (c) 2015 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 12/15141-6 - Screening Er:YAG (SSP) laser parameters to increase longitudinal adhesion to caries affected dentine
• Grantee: Adriana Bona Matos
• Support Opportunities: Regular Research Grants