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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

DNA Methylation Changes Induced by a High-Fat Diet and Fish Oil Supplementation in the Skeletal Muscle of Mice

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Amaral, Catia L. [1, 2] ; Crisma, Amanda R. [1] ; Masi, Laureane N. [1] ; Martins, Amanda R. [1] ; Hirabara, Sandro M. [3] ; Curi, Rui [1]
Total Authors: 6
[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508000 Sao Paulo, SP - Brazil
[2] State Univ Goias, Anapolis - Brazil
[3] Univ Cruzeiro Sul, Inst Phys Act Sci & Sports, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Nutrigenetics and Nutrigenomics; v. 7, n. 4-6, p. 314-326, 2014.
Web of Science Citations: 8

Background/Aims: To investigate the global changes in DNA methylation and methylation of the promoter region of the peroxisome proliferator-activated receptor gamma transcript variant 2 (Pparg2) gene resulting from a high-fat diet (HFD) and/or fish oil supplementation. Methods: Fish oil, rich in omega-3 polyunsaturated fatty acids, or water was orally administered to male mice for 12 weeks. After the first 4 weeks, the animals were fed a control diet or an HFD until the end of the experimental protocol, when the epididymal fat, gastrocnemius muscle and liver were excised. Results: Pparg2 mRNA expression was upregulated by obesity and downregulated by fish oil supplementation in the liver. In the gastrocnemius muscle, diet-induced obesity increased global DNA methylation. Fish oil prevented the decrease in Pparg2 promoter methylation induced by obesity in the gastrocnemius muscle. Regardless of the diet given, fish oil supplementation increased Pparg2 promoter methylation at CpG-263 in muscle and adipose tissue. Conclusion: HFD and fish oil modified global and Pparg2 promoter DNA methylation in a tissue-specific manner. Fish oil supplementation attenuated body weight gain, abolished the increase in Pparg2 expression in the liver and prevented the decrease in Pparg2 promoter methylation in the muscle induced by the HFD. (C) 2015 S. Karger AG, Basel (AU)

FAPESP's process: 10/07596-8 - Evaluation of DNA methylation changes by high-fat-induced obesity and a possible modulated efect of omega-3 fatty acids in vivo
Grantee:Cátia Lira Do Amaral
Support type: Scholarships in Brazil - Post-Doctorate