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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diversity of human papillomavirus in the anal canal of men: the HIM Study

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Sichero, L. [1] ; Nyitray, A. G. [2] ; Nunes, E. M. [1] ; Nepal, B. [2] ; Ferreira, S. [1] ; Sobrinho, J. S. [1] ; Baggio, M. L. [1] ; Galan, L. [3] ; Silva, R. C. [4] ; Lazcano-Ponce, E. [5] ; Giuliano, A. R. [6] ; Villa, L. L. [1, 7, 8] ; Grp, HIM Study
Total Authors: 13
[1] ICESP, Mol Biol Lab, Ctr Translat Oncol, Sao Paulo - Brazil
[2] Univ Texas Houston, Sch Publ Hlth, Ctr Infect Dis, Houston, TX - USA
[3] Ludwig Inst Canc Res, Heidelberg, Vic - Australia
[4] Ctr Referencia & Treinamento DST Aids, Sao Paulo - Brazil
[5] Inst Nacl Salud Publ, Ctr Invest Salud Poblac, Cuernavaca, Morelos - Mexico
[6] H Lee Moffitt Canc Ctr & Res Inst, Ctr Infect Res Canc, Tampa, FL - USA
[7] Univ Sao Paulo, Dept Radiol & Oncol, Sch Med, Santa Casa De Sao Paulo - Brazil
[8] HPV Inst, Sch Med, Santa Casa De Sao Paulo - Brazil
Total Affiliations: 8
Document type: Journal article
Source: Clinical Microbiology and Infection; v. 21, n. 5, p. 502-509, MAY 2015.
Web of Science Citations: 10

Human papillomavirus (HPV) infections are associated with the development of anogenital lesions in men. There are no reports describing the distribution of non-alpha HPV types in the anal canal of a sexually diverse group of men. The HPV Infection in Men (HIM) Study is a multicentre study on the natural history of HPV infection in Brazil, Mexico, and the USA. At baseline, 12% of anal canal PCR HPV-positive specimens were not typed by the Roche Linear Array, and were considered to be unclassified. Our goals were to characterize HPVs among these unclassified specimens at baseline, and to assess associations with participant socio-demographic and behavioural characteristics. Unclassified HPVs were typed by sequencing of amplified PGMY09/11 products or cloning of PGMY/GP + nested amplicons followed by sequencing. Further analysis was conducted with FAP primers. Of men with unclassified HPV in the anal canal, most (89.1%) were men who have sex with women. Readable sequences were produced for 62.8% of unclassified specimens, of which 75.2% were characterized HPV types. Eighteen, 26 and three different alpha-HPV, beta-HPV and gamma-HPV types were detected, respectively. alpha-HPVs were more commonly detected among young men (18-30 years) than among older men (45-70 years), whereas beta-HPVs were more frequent among mid-adult men (31-44 years). beta-HPVs were more common among heterosexual men (85.0%) than among non-heterosexual Men. All beta-HPVs detected among non-heterosexual men were beta 2-HPV types. The high prevalence of beta-HPV in the anal canal of men who do not report receptive anal sex is suggestive of other forms of transmission that do not involve penile anal intercourse. Clinical Microbiology and Infection (C) 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 08/57889-1 - Institute of Science and Technology to study Diseases Associated with Papillomavirus
Grantee:Luisa Lina Villa
Support type: Research Projects - Thematic Grants
FAPESP's process: 10/15282-3 - Diversity of HPV types in the male genital region
Grantee:Laura Cristina Sichero Vettorazzo
Support type: Regular Research Grants