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Prevalence and risk factors associated to Beta-papillomavirus detection in anogenital and oral samples of males from the HIM study

Grant number: 15/12557-5
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): October 10, 2015
Effective date (End): November 09, 2015
Field of knowledge:Biological Sciences - Biochemistry - Biochemistry of Microorganisms
Principal Investigator:Laura Cristina Sichero Vettorazzo
Grantee:Emily Montosa Nunes
Supervisor abroad: Anna R. Giuliano
Home Institution: Instituto do Câncer do Estado de São Paulo Octavio Frias de Oliveira (ICESP). Coordenadoria de Serviços de Saúde (CSS). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Local de pesquisa : Moffitt Cancer Center, United States  
Associated to the scholarship:13/20470-1 - Prevalence and diversity of Beta-papillomavirus in anogenital and oral samples of males from the him study, BP.MS

Abstract

HPV is primarily transmitted by sexual contact and infection by these viruses is strongly associated with the development of tumors in the cervix, vulva and anus in women, cancer of the penis and anus in men, as well as tumors in the head and neck in both genders. Case-control studies conducted in women with cervical cancer showed that male partners' sexual behavior influences the risk of women of developing this neoplasia. However little is known about the natural history of HPV infection in the male genital region. Since 2005 an International prospective study in men (HPV Infection in Men - HIM, Brazil, Mexico, United States) is being conducted following-up approximately 4,500 men every 6 months for 4 years. For viral genotyping of the samples, a methodology capable of identifying 37 HPV types commonly detected in female genital samples is being used. Initial analysis of genital samples from the HIM study revealed an HPV prevalence of 65.2%, of which 14.7% categorizes as unclassified infections, i.e. without positive type defined. Among these non-classified scrotum/penile and anal samples, high prevalence of cutaneous HPV, mainly Beta-HPV types, was further observed using a PCR-sequencing protocol. In addition, genital samples were analyzed using a bead-based multiplex genotyping methodology and multiple infections by different Beta-HPV types were unraveled in most specimens suggesting that the PCR-sequencing protocol used may be underestimating the real prevalence of "cutaneous" HPV types among these smears. In order to better understand epidemiological significances of these viral types in the natural history of HPV infection, our aim is: (1) To determine the frequency of individual Beta-HPV types detected among 800 matched oral/penile/anal samples; (2) To identify factors independently associated with detection of these HPV types in different anatomical sites of men participating in the HIM study. Differences in socio-demographic and behavioral risk factors considered being associated with the overall presence of ²-HPV, and of specific ²-HPV genus and types will be evaluated. Epidemiological analysis will be based on data obtained by Luminex technology in São Paulo. Epidemiological analysis we be conducted using the SAS® software. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NUNES, EMILY M.; LOPEZ, ROSSANA V. M.; SUDENGA, STACI L.; GHEIT, TARIK; TOMMASINO, MASSIMO; BAGGIO, MARIA L.; FERREIRA, SILVANEIDE; GALAN, LENICE; SILVA, ROBERTO C.; LAZCANO-PONCE, EDUARDO; GIULIANO, ANNA R.; VILLA, LUISA L.; SICHERO, LAURA; GRP, HIM STUDY. Concordance of Beta-papillomavirus across anogenital and oral anatomic sites of men: The HIM Study. VIROLOGY, v. 510, p. 55-59, OCT 2017. Web of Science Citations: 7.
NUNES, EMILY MONTOSA; SUDENGA, STACI L.; GHEIT, TARIK; TOMMASINO, MASSIMO; BAGGIO, MARIA LUIZA; FERREIRA, SILVANEIDE; GALAN, LENICE; SILVA, ROBERTO C.; CAMPBELL, CHRISTINE M. PIERCE; LAZCANO-PONCE, EDUARDO; GIULIANO, ANNA R.; VILLA, LUISA L.; SICHERO, LAURA; GRP, HIM STUDY. Diversity of beta-papillomavirus at anogenital and oral anatomic sites of men: The HIM Study. VIROLOGY, v. 495, p. 33-41, AUG 2016. Web of Science Citations: 22.

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