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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genotoxic effect of photodynamic therapy mediated by curcumin on Candida albicans

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Author(s):
Carmello, Juliana Cabrini [1] ; Pavarina, Ana Claudia [1] ; Oliveira, Rui [2] ; Johansson, Bjoern [3]
Total Authors: 4
Affiliation:
[1] Sao Paulo State Univ UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil
[2] Univ Minho, Dept Biol, Ctr Res & Technol Agroenvironm & Biol Sci CITAB, P-4710057 Braga - Portugal
[3] Univ Minho, Dept Biol, Ctr Mol & Environm Biol CBMA, P-4710057 Braga - Portugal
Total Affiliations: 3
Document type: Journal article
Source: FEMS Yeast Research; v. 15, n. 4 JUN 2015.
Web of Science Citations: 9
Abstract

Photodynamic therapy (PDT) is a promising method for localized and specific inactivation of fungi and bacteria. A nontoxic light-sensitive compound is taken up by cells, which are then exposed selectively to light, which activates toxicity of the compound. We investigated the potential of sublethal PDT using light-sensitive curcumin (CUR) in combination with blue (455 nm) light to promote reactive oxygen species (ROS) formation in the form of singlet oxygen and DNA damage of Candida albicans. Surprisingly, CUR-mediated PDT but also light alone caused significantly longer comet tails, an indication of DNA damage of C. albicans when compared with the negative control. The intracellular ROS production was also significantly higher for the group treated only with light. However, PDT compared to blue light alone significantly slowed DNA repair. Comet tails decreased during 30 min visualized as a 90% reduction in length in the absence of light for cells treated with light alone, while comet tails of cells treated with PDT only diminished in size about 45%. These results indicate that complex mechanisms may result in PDT in a way that should be considered when choosing the photosensitive compound and other aspects of the treatment design. (AU)

FAPESP's process: 12/17468-2 - Effect of Antimicrobial Photodynamic Therapy in a murine model of oral candidosis. In vivo evaluation of a Photodithazine hydrogel and Aluminum-Chlorine-Phthalocyanine cationic nanoemulsion.
Grantee:Juliana Cabrini Carmello
Support Opportunities: Scholarships in Brazil - Doctorate