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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Stress tolerance and virulence of insect-pathogenic fungi are determined by environmental conditions during conidial formation

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Author(s):
Rangel, Drauzio E. N. [1, 2] ; Braga, Gilberto U. L. [3] ; Fernandes, Everton K. K. [1, 4] ; Keyser, Chad A. [5] ; Hallsworth, John E. [6] ; Roberts, Donald W. [1]
Total Authors: 6
Affiliation:
[1] Utah State Univ, Dept Biol, Logan, UT 84322 - USA
[2] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[4] Univ Fed Goias, Inst Patol Trop & Saude Publ, BR-74605050 Goiania, Go - Brazil
[5] Univ Copenhagen, Dept Plant & Environm Sci, DK-1871 Frederiksberg - Denmark
[6] Queens Univ Belfast, Inst Global Food Secur, Sch Biol Sci, MBC, Belfast BT9 7BL, Antrim - North Ireland
Total Affiliations: 6
Document type: Journal article
Source: CURRENT GENETICS; v. 61, n. 3, p. 383-404, AUG 2015.
Web of Science Citations: 62
Abstract

The virulence to insects and tolerance to heat and UV-B radiation of conidia of entomopathogenic fungi are greatly influenced by physical, chemical, and nutritional conditions during mycelial growth. This is evidenced, for example, by the stress phenotypes of Metarhizium robertsii produced on various substrates. Conidia from minimal medium (Czapek's medium without sucrose), complex medium, and insect (Lepidoptera and Coleoptera) cadavers had high, moderate, and poor tolerance to UV-B radiation, respectively. Furthermore, conidia from minimal medium germinated faster and had increased heat tolerance and were more virulent to insects than those from complex medium. Low water-activity or alkaline culture conditions also resulted in production of conidia with high tolerance to heat or UV-B radiation. Conidia produced on complex media exhibited lower stress tolerance, whereas those from complex media supplemented with NaCl or KCl (to reduce water activity) were more tolerant to heat and UV-B than those from the unmodified complex medium. Osmotic and nutritive stresses resulted in production of conidia with a robust stress phenotype, but also were associated with low conidial yield. Physical conditions such as growth under illumination, hypoxic conditions, and heat shock before conidial production also induced both higher UV-B and heat tolerance; but conidial production was not decreased. In conclusion, physical and chemical parameters, as well as nutrition source, can induce great variability in conidial tolerance to stress for entomopathogenic fungi. Implications are discussed in relation to the ecology of entomopathogenic fungi in the field, and to their use for biological control. This review will cover recent technologies on improving stress tolerance of entomopathogenic fungi for biological control of insects. (AU)

FAPESP's process: 12/15204-8 - Mechanistic study of the photodynamic inactivation of human and plant pathogenic fungi
Grantee:Gilberto Úbida Leite Braga
Support type: Regular Research Grants
FAPESP's process: 14/01229-4 - International Symposium on Fungal Stress - ISFUS
Grantee:Drauzio Eduardo Naretto Rangel
Support type: Research Grants - Organization of Scientific Meeting
FAPESP's process: 10/06374-1 - Visible light during growth enhances conidial tolerance to different stress conditions in fungi
Grantee:Drauzio Eduardo Naretto Rangel
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/50518-6 - Stress related genes are induced by visible light during mycelial growth resulting in increased conidial tolerance to stress conditions
Grantee:Drauzio Eduardo Naretto Rangel
Support type: Regular Research Grants