Genetic Effects of eNOS Polymorphisms on Biomarkers Related to Cardiovascular Status in a Population Coexposed to Methylmercury and Lead

Author(s)

Mazzaron Barcelos, Gustavo Rafael ^[1] ; De Marco, Katia Cristina ^[1] ; de Rezende, Vania Braghini ^[1] ; Leite Braga, Gilberto Ubida ^[1] ; Greggi Antunes, Lusania Maria ^[1] ; Tanus-Santos, Jose Eduardo ^[2] ; Barbosa, Jr., Fernando ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Analyses Toxicol & Food Sci, BR-14040903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pharmacol, BR-14049900 Sao Paulo - Brazil

• Document type: Journal article
• Source: Archives of Environmental Contamination and Toxicology; v. 69, n. 2, p. 173-180, AUG 2015.
• Web of Science Citations: 2

Abstract

The aim of the present study was to evaluate possible effects of endothelial nitric oxide synthase (eNOS) polymorphisms on systolic (SBP) and diastolic blood pressure (DBP) and on nitrite levels in plasma (NitP) in a population coexposed to methylhemoglobin (MeHg) and lead (Pb) in the Amazonian region, Brazil. Plasmatic levels of hemoglobin Hg (HgP) and Pb (PbP) were determined by inductively coupled plasma-mass spectrometry, whereas NitP were quantified by chemiluminescence. Genotyping was performed by conventional and restriction fragment length polymorphism-polymerase chain reaction assay. The population age ranged from 18 to 87 years (mean 40 +/- A 16), and the distribution between the sexes was homogenous (63 men and 50 women). Mean HgP and PbP were 7.1 +/- A 6.1 and 1.1 +/- A 1.1 A mu g L-1, respectively. PbP was correlated to SBP and DBP, whereas no effects were observed for HgP on blood pressure. Subjects carrying the 4b allele in intron 4 presented greater SBP and DBP compared with those who had the 4a4a genotype. In addition, interactions between alcohol consumption and the -786 T/C polymorphism were observed on NitP, i.e., individuals carrying the polymorphic allele and drinkers had lower NitP. Taken together, our data give new insights concerning the genetic effects of eNOS polymorphisms on biomarkers related to cardiovascular status in populations coexposed to Hg and Pb. (AU)

• FAPESP's process: 11/22575-0 - Evaluation of polymorphisms genes related to glutathione on the mercury concentrations in individuals environmentally exposed to the metal, in riparian communities of Tapajos river, Pará, Brazil
• Grantee: Gustavo Rafael Mazzaron Barcelos
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 10/16691-4 - Evaluation of mercury and lead metabolism and toxicity related polymorphisms in riverside communities exposed to the metals, in Amazonian region
• Grantee: Gustavo Rafael Mazzaron Barcelos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral