Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

New glycosylated conjugate copolymer N-acetyl-beta-D-glucosaminyl-pluronic: Synthesis, self-assembly and biological assays

Full text
Show less -
Allievi Frizon, Tiago Elias [1] ; Serafini Micheletto, Yasmine Miguel [1] ; Westrup, Jose Luiz [1] ; Silva Wakabayashi, Priscila Sayoko [1] ; Serafim, Francieli Rocha [1] ; Damiani, Adriani Paganini [2] ; Longaretti, Luiza Martins [2] ; de Andrade, Vanessa Moraes [2] ; Giacomelli, Fernando Carlos [3] ; Fort, Sebastien [4] ; Dal Bo, Alexandre Goncalves [1, 4]
Total Authors: 11
[1] Univ Extremo Sul Catarinense, Lab Processamento Polimeros Avancados LAPPA, BR-88806000 Criciuma, SC - Brazil
[2] Univ Extremo Sul Catarinense, PPGCS, Lab Biol Celular & Mol, BR-88806000 Criciuma, SC - Brazil
[3] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre, SP - Brazil
[4] Univ Grenoble Alpes, CERMAV, F-38000 Grenoble - France
Total Affiliations: 4
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 133, p. 323-330, SEP 1 2015.
Web of Science Citations: 2

This work describes the synthesis of a new glycosylated conjugate copolymer, GlcNAc-PEO75-PPO30-PEO75-GlcNAc (GlcNAc-PluronicF68-GIcNAc), using click chemistry from Pluronic (R) F68 and propargyl-2-N-acetamido-2-deoxy-beta-D-glucopyranoside. Micelles were prepared by the self-assembly of GlcNAc-PluronicF68-GlcNAc in phosphate-buffered solution. The critical micelle concentration was determined by fluorescence spectroscopy, and the value was found to be equal to 5.8 mg mL(-1). The Gibbs free energy (Delta G) of micellization is negative, indicating that the organization of amphiphiles is governed by the hydrophobic effects in an entropy-driven process. The scattering characterization of GlcNAc-PluronicF68-GlcNAc micelles showed a hydrodynamic radius of 8.7 nm and negative zeta potential (-21.0 +/- 0.9 mV). The TEM image evidences the spherical shape of the objects self-assemble into highly regular micelles having a mean diameter of 10 nm. The SAXS profile confirmed the spherical shape of the assemblies comprising a swollen PPO core (R-core = 2.25 nm) stabilized by PEO chains following Gaussian statistics. The results of the comet assay showed that the GlcNAc-PluronicF68-GlcNAc micelles were not genotoxic, and the cell viability test was higher than 97% for all concentrations, demonstrating that GlcNAc-PluronicF68-GlcNAc is not toxic. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/14087-8 - Development of size-tuned polymeric nanoparticles and evaluation of the interaction with biological environments
Grantee:Fernando Carlos Giacomelli
Support Opportunities: Regular Research Grants