Immobilization of Fusarium graminearum beta-D-fructofuranosidase using alternative cellulosic supports: Stabilization and production of fructooligosaccharides

Author(s)

Goncalves, Heloisa Bressan ^[1] ; Jorge, Joao Atilio ^[2] ; Souza Guimaraes, Luis Henrique ^{[2, 1]}

Affiliation

^[1] Univ Estadual Paulista, Inst Quim Araraquara, BR-14800900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Sao Paulo - Brazil

• Document type: Journal article
• Source: FOOD SCIENCE AND BIOTECHNOLOGY; v. 24, n. 4, p. 1429-1435, AUG 2015.
• Web of Science Citations: 2

Abstract

The extracellular beta-d-fructofuranosidase from Fusarium graminearum was immobilized using hydrophilic cotton, filter paper, multipurpose cloth, sugar cane bagasse, string, or gauze as alternative cellulosic supports, or with cyanogen bromide agarose. All derivatives (support+enzyme) showed high capacity for reuse (up to 23 times). The derivatives obtained with multipurpose cloth and string were stable at 60A degrees C maintaining 80% of their activity for more than 120 min. The filter paper derivative had a halflife (T-50) of 27 min at 70A degrees C. When tested for their pH stability (3.0-9.0), all derivatives were more stable than the free enzyme, especially the cotton derivative. The sugarcane bagasse, string, and filter paper derivatives could efficiently produce fructooligosaccharides (FOS) using sucrose as substrate. According to the retention of enzymatic activity, reuse and stabilities, the filter paper and string were the best alternative supports for beta-d-fructofuranosidase immobilization, allowing enzyme stabilization and production of FOS. (AU)