Evaluating the microbicidal, antiparasitic and antitumor effects of CR-LAAO from Calloselasma rhodostoma venom

Author(s)

Costa, Tassia R. ^[1] ; Menaldo, Danilo L. ^[1] ; da Silva, Cassio Prinholato ^[1] ; Sorrechia, Rodrigo ^[2] ; de Albuquerque, Sergio ^[1] ; Pietro, Rosemeire C. L. R. ^[2] ; Ghisla, Sandro ^[3] ; Antunes, Lusania M. Greggi ^[1] ; Sampaio, Suely V. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, BR-14040903 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci Araraquara, Dept Drugs & Med, Araraquara, SP - Brazil

^[3] Univ Konstanz, Dept Biol, Constance - Germany

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 80, p. 489-497, SEP 2015.
• Web of Science Citations: 23

Abstract

CR-LAAO is an L-amino acid oxidase from Calloselasma rhodostoma snake venom that has been broadly studied regarding its structural and biochemical characteristics, however, few studies have investigated its pharmacological effects. The present study aimed at the evaluation of the biotechnological potential of CR-LAAO by determining its bactericidal, antifungal, leishmanicidal and trypanocidal activity, as well as its cytotoxicity on human tumor and non-tumor cell lines. After 24 h of preincubation, CR-LAAO showed bactericidal effects against both Staphylococcus aureus (MIC 0.78 mu g/mL) and Escherichia coli (MIC 31.25 mu g/mL) strains, inducing dismantle of bacterial cell walls. After 6 h of preincubation with Candida albicans, CR-LAAO was able to inhibit 80% of the yeast growth, and it also showed cytotoxic activity on Leishmania species and Trypanosoma cruzi. Additionally, CR-LAAO showed high cytotoxicity on HepG2 and HL-60 tumor cells (IC50 10.78 and 1.7 mu g/mL), with lower effects on human mononuclear cells (PBMC). The cytotoxic effects of CR-LAAO were significantly inhibited in the presence of catalase, which suggests the involvement of hydrogen peroxide in its mechanisms of toxicity. Therefore, CR-LAAO showed promising pharmacological effects, and these results provide important information for the development of therapeutic strategies with directed action, such as more effective antimicrobial agents. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
• Grantee: Suely Vilela
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/02645-3 - Functional characterization and mechanisms responses from damage caused by L-aminoacid oxidase from Calloselasma rhodostoma in human cell lines
• Grantee: Tássia Rafaella Costa
• Support Opportunities: Scholarships in Brazil - Doctorate