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Evaluation of genotoxic potential of an isolated L-amino acid oxidase from Calloselasma rhodostoma (CR-LAAO) venom on human cell lines

Grant number: 15/00847-9
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Suely Vilela
Grantee:Martin Krahenbuhl Amstalden
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis, AP.TEM


The L-amino acid oxidases isolated and characterized from snake venom (SV-LAAOs) have many biological and pharmacological effects, such as induction of apoptosis, cytotoxicity, hemorrhage, hemolysis, edema, bactericidal and anti-parasitic activities. The CR-LAAO is an L-amino acid oxidase isolated from Calloselasma rhodostoma snake venom, which was isolated and characterized by Ponnudurai et al. (1994). There are several studies with this protein in relation to its structural features and part of its functionality. However, studies on the genotoxic effects that the protein can cause in the human body have not yet been described. The comet assay, which detects single and double strand breaks of DNA and alkali-labile sites, will be used to elucidate the mechanisms of action of this toxin on the genomic instability. The results may contribute to the elucidation of the mechanisms of action of CR-LAAO and its possible application in the biomedical field.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COSTA, TASSIA R.; AMSTALDEN, MARTIN K.; RIBEIRO, DIEGO L.; MENALDO, DANILO L.; SARTIM, MARCO A.; AISSA, ALEXANDRE F.; ANTUNES, LUSANIA M. G.; SAMPAIO, V, SUELY. CR-LAAO causes genotoxic damage in HepG2 tumor cells by oxidative stress. Toxicology, v. 404, p. 42-48, . (15/00740-0, 11/23236-4, 15/00847-9)

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