Antibacterial and anti-inflammatory activities of an extract, fractions, and compounds isolated from Gochnatia pulchra aerial parts

Author(s)

Lucarini, R. ^[1] ; Tozatti, M. G. ^[1] ; Silva, M. L. A. ^[1] ; Gimenez, V. M. M. ^[2] ; Pauletti, P. M. ^[1] ; Groppo, M. ^[3] ; Turatti, I. C. C. ^[4] ; Cunha, W. R. ^[1] ; Martins, C. H. G. ^[1]

Affiliation

^[1] Univ Franca, Nucleo Pesquisas Ciencias Exatas & Tecnol, Franca, SP - Brazil

^[2] Ctr Univ Claretiano, Batatais, SP - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Medical and Biological Research; v. 48, n. 9, p. 822-830, SEP 2015.
• Web of Science Citations: 5

Abstract

This paper reports on the in vitro antibacterial and in vivo anti-inflammatory properties of a hydroethanolic extract of the aerial parts of Gochnatia pulchra (HEGP). It also describes the antibacterial activity of HEGP fractions and of the isolated compounds genkwanin, scutellarin, apigenin, and 3,5-O-dicaffeoylquinic acid, as evaluated by a broth microdilution method. While HEGP and its fractions did not provide promising results, the isolated compounds exhibited pronounced antibacterial activity. The most sensitive microorganism was Streptococcus pyogenes, with minimum inhibitory concentration (MIC) values of 100, 50 and 25 µg/mL for genkwanin and the flavonoids apigenin and scutellarin, respectively. Genkwanin produced an MIC value of 25 µg/mL against Enterococcus faecalis. A paw edema model in rats and a pleurisy inflammation model in mice aided investigation of the anti-inflammatory effects of HEGP. This study also evaluated the ability of HEGP to modulate carrageenan-induced interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) production. Orally administered HEGP (250 and 500 mg/kg) inhibited carrageenan-induced paw edema. Regarding carrageenan-induced pleurisy, HEGP at 50, 100, and 250 mg/kg diminished leukocyte migration by 71.43%, 69.24%, and 73.34% (P<0.05), respectively. HEGP suppressed IL-1β and MCP-1 production by 55% and 50% at 50 mg/kg (P<0.05) and 60% and 25% at 100 mg/kg (P<0.05), respectively. HEGP abated TNF-α production by macrophages by 6.6%, 33.3%, and 53.3% at 100, 250, and 500 mg/kg (P<0.05), respectively. HEGP probably exerts anti-inflammatory effects by inhibiting production of the pro-inflammatory cytokines TNF-α, IL-1β, and MCP-1. (AU)

• FAPESP's process: 12/16182-8 - Chemical study and biological assays with species of the cerrado searching to antiparasitic substances
• Grantee: Wilson Roberto Cunha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/02066-0 - Prospecting of biological chemistry Gochnatia pulchra in the development potential of antibacterial, anti-inflammatory and antiparasitic agents
• Grantee: Rodrigo Lucarini
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 08/07329-0 - Bioprospection for antimicrobial and anti-inflammatory agents of the specie vegetable Gochnatia pulchra
• Grantee: Rodrigo Lucarini
• Support Opportunities: Scholarships in Brazil - Master