| Full text | |
| Author(s): |
Total Authors: 4
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| Affiliation: | [1] Univ Estadual Paulista, Inst Biociencias, Dept Zool, Rio Claro, SP - Brazil
[2] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands - England
Total Affiliations: 2
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| Document type: | Journal article |
| Source: | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY; v. 188, p. 17-21, OCT 2015. |
| Web of Science Citations: | 9 |
| Abstract | |
Measurement of heart rate (f(H)) in embryonic reptiles has previously imposed some degree of invasive treatment on the developing embryo. Recently a non-invasive technique of f(H) detection from intact eggs was developed for commercial avian breeders and has since been used in biological research. This device uses infrared light, enabling it to detect heartbeats in very early embryos. However, infrared light is a source of heat and extended enclosure of an egg in the device is likely to affect temperature with consequent effects on physiological processes, including f(H). We studied the effect of use of the monitor on the temperature of eggs and on fH in two species of reptiles, the snapping turtle (Chelydra serpentina) and the green iguana (Iguana iguana). Egg temperature increased from a room temperature of 27-28 degrees C, by 26% in turtles and 14% in iguanas over 1 h of enclosure, resulting in an increase in f(H) of 76-81% in turtles and 35-50% iguanas. These effects on f(H) can either be avoided by brief enclosure of each egg in the monitor or measured and accounted for during the design of long-term experiments. (C) 2015 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 12/16537-0 - Cardiovascular autonomic control and metabolism in lizard embryos (Reptilia; Lepidosauria) |
| Grantee: | Marina Rincon Sartori |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/06938-8 - Development of neural control of the cardiovascular system in reptiles |
| Grantee: | Augusto Shinya Abe |
| Support Opportunities: | Research Grants - Visiting Researcher Grant - International |